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Characterization Of Elastin-Like Polypeptides Using Viscometry, Hamdan Noman Alanazi
Characterization Of Elastin-Like Polypeptides Using Viscometry, Hamdan Noman Alanazi
ETD Archive
Elastin-like polypeptides (ELPs) are a class of polypeptide polymers that are gaining interest in various potential applications. These polymers are responsive to the changes in their environment by exhibiting conformational changes and aggregation. Monodisperse elastin-like polypeptides, (GVGVP)40, (GVGVP)40-foldon, and (GVGVP)60-foldon are made in bacterial expression system. An Ubbelohde capillary viscometer was set up to characterize the structural changes of these ELPs in phosphate buffered saline (PBS) solution. For the ELP-foldon, the relative viscosity measurements were utilized to calculate the intrinsic viscosities using Kraemer and Huggins equations. The known molecular weights of the ELPs and the experimentally determined intrinsic viscosities facilitated …