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Spatial Heterogeneity Analysis In Evaluation Of Cell Viability And Apoptosis For Colorectal Cancer Cells, Aydin Saribudak, Herman Kucharavy, Karen Hubbard, Muharrem Umit Uyar
Spatial Heterogeneity Analysis In Evaluation Of Cell Viability And Apoptosis For Colorectal Cancer Cells, Aydin Saribudak, Herman Kucharavy, Karen Hubbard, Muharrem Umit Uyar
Publications and Research
In evaluation of cell viability and apoptosis, spatial heterogeneity is quantified for cancerous cells cultured in 3-D in vitro cell-based assays under the impact of anti-cancer agents. In 48-h experiments using human colorectal cancer cell lines of HCT-116, SW-620, and SW-480, incubated cells are divided into control and drug administered groups, to be grown in matrigel and FOLFOX solution, respectively. Our 3-D cell tracking and data acquisition system guiding an inverted microscope with a digital camera is utilized to capture bright field and fluorescent images of colorectal cancer cells at multiple time points. Identifying the locations of live and dead …