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Advancing Cell-Free Protein Synthesis Systems For On-Demand Next-Generation Protein Therapeutics And Clinical Diagnostics, Emily Ann Long Zhao
Advancing Cell-Free Protein Synthesis Systems For On-Demand Next-Generation Protein Therapeutics And Clinical Diagnostics, Emily Ann Long Zhao
Theses and Dissertations
Recombinant proteins have many medical and industrial applications, but their use is complicated by commercial production and stability constraints. These issues are particularly challenging for recombinant proteins used in pharmaceutical therapeutics and clinical diagnostics. Expensive production and distribution limit the accessibility of therapeutics and diagnostics especially in the developing world. Additionally, clinical use of recombinant proteins face further challenges within biological systems including biological degradation and immunogenicity. To increase the accessibility of recombinant proteins, the cost and inefficiencies of protein manufacturing and distribution need to be significantly reduced. A powerful tool to aid in this endeavor is cell-free protein synthesis …
Designing Cell-Free Protein Synthesis Systems For Improved Biocatalysis And On-Demand, Cost-Effective Biosensors, Mehran Soltani Najafabadi
Designing Cell-Free Protein Synthesis Systems For Improved Biocatalysis And On-Demand, Cost-Effective Biosensors, Mehran Soltani Najafabadi
Theses and Dissertations
The open nature of Cell-Free Protein Synthesis (CFPS) systems has enabled flexible design, easy manipulation, and novel applications of protein engineering in therapeutic production, biocatalysis, and biosensors. This dissertation reports on three advances in the application of CFPS systems for 1) improving biocatalysis performance in industrial applications by site-specific covalent enzyme immobilization, 2) expressing and optimizing a difficult to express a mammalian protein in bacterial-based CFPS systems and its application for cost-effective, on-demand biosensors compatible with human body fluids, and 3) streamlining the procedure of an E. coli extract with built-in compatibility with human body fluid biosensors. Site-specific covalent immobilization …