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Advancing Cell-Free Protein Synthesis Systems For On-Demand Next-Generation Protein Therapeutics And Clinical Diagnostics, Emily Ann Long Zhao
Advancing Cell-Free Protein Synthesis Systems For On-Demand Next-Generation Protein Therapeutics And Clinical Diagnostics, Emily Ann Long Zhao
Theses and Dissertations
Recombinant proteins have many medical and industrial applications, but their use is complicated by commercial production and stability constraints. These issues are particularly challenging for recombinant proteins used in pharmaceutical therapeutics and clinical diagnostics. Expensive production and distribution limit the accessibility of therapeutics and diagnostics especially in the developing world. Additionally, clinical use of recombinant proteins face further challenges within biological systems including biological degradation and immunogenicity. To increase the accessibility of recombinant proteins, the cost and inefficiencies of protein manufacturing and distribution need to be significantly reduced. A powerful tool to aid in this endeavor is cell-free protein synthesis …
Engineering Cell-Free Protein Expression Systems For Biotherapeutics And Biosensing, John Porter Hunt
Engineering Cell-Free Protein Expression Systems For Biotherapeutics And Biosensing, John Porter Hunt
Theses and Dissertations
Therapeutic proteins have become a cornerstone of modern medicine since the FDA approval of recombinant human insulin in 1982. Likewise, biosensors transform chemical detection and disease diagnostics by identifying biomarkers, chemical contaminants, and infective agents. Long-standing methods for creating therapeutics and biosensors employ whole cells such as Escherichia coli (E. coli). Alternatively, cell-free protein synthesis (CFPS) employs the enzymatic reactions necessary for protein production and biosensing within a cell, but in an engineered reactor environment facilitating unprecedented access to and control over biochemical machinery, preservation by cryodesiccation for portable deployment, and functionality in cytotoxic applications. This dissertation reports advances in …