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Theses

1988

Biosensors.

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Design Of A Fiber Optic Oxygen Sensor Based On Immobilized Hemoglobin, Mendu Govind Raj May 1988

Design Of A Fiber Optic Oxygen Sensor Based On Immobilized Hemoglobin, Mendu Govind Raj

Theses

The fiber optic Oxygen sensor consists of a thin layer of immobilized deoxyhemoglobin at the common end of a bifurcated fiber. Immobilization is done using the alginate gel procedure. The immobilized reagent is separated from the standard oxygen sample by the gel with or without semi-permeable membrane, and the entire configuration is enclosed in an oxygen free sample cell. Light from a tungsten-halogen lamp, filtered through a 405nm filter is passed through one arm of a bifurcated fiber to the sample cell. The reflected intensity (a highly polished aluminum foil or a plate is placed at the bottom of the …