Engineering Commons^™

Technological Aspects Of Molecular Diagnosis Of Bacterial Infectious Diseases, Christine S. Booth

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

The polymerase chain reaction (PCR) is continually growing in its application, particularly in the field of molecular diagnosis of disease from clinical specimens. The main focus has been in the detection and identification of pathogens. However, quantitative PCR is increasingly utilized to determine initial pathogen load. A well-designed PCR protocol is required in all of these instances. Just as importantly, in the context of disease diagnosis; is the design of the sample processing methodology. The ideal method should concentrate the DNA and effectively isolate a high-quality DNA product, free of PCR inhibitors, while also being simple, reproducible and safe.

The …

Numerical Simulation Of Buoyancy-Driven Turbulent Ventilation In Attic Space Under Winter Conditions, Shimin Wang, Zhigang Shen, Linxia Gu

Department of Mechanical and Materials Engineering: Faculty Publications

Attic design and construction have significant impacts on residential buildings’ energy performance. In order to understand how passive ventilation rates affect ridge-vent attic’s performance, a two-dimensional steady-state finite volume model is employed to simulate the buoyancy-driven turbulent ventilation and heat transfer in a triangular attic space of a gable-roof residential building under winter conditions. The modeled attic has a pitch of 5/12 and a passive ventilation system, consisting of continuous ridge and soffit vents. The v2f model is used to analyze the turbulent air flow and natural convection heat transfer inside the attic. The effects of ambient air temperature, vent …