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Inside-Out Crosslinked And Commercial-Scale Hydrogels, And Sub-Macromolecular Selective Purification Using The Hyrdogels, William H. Velander, Kevin E. Van Cott

Department of Chemical and Biomolecular Engineering: Patents

Relating to chromatographic processes and ion-exchange and affinity matrices, a spatial installation method for a bifunctional reagent that crosslinks and/or activates a polymer matrix is disclosed, with inside-outside installation of a bifunctional reagent on and within a polymer matrix. The polymer matrix is cellulose, agarose, or chitosan particles. The installation may be followed by inside-outside ligand attachment, by further reacting the matrix with a ligand or ionic group so that a higher concentration of ligand or ionic moiety occurs on the intra-particle volume than the outer matrix surface.