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Cellulose-Specific Type B Carbohydrate Binding Modules: Understanding Oligomeric And Non-Crystalline Substrate Recognition Mechanisms, Abhishek A. Kognole, Christina M. Payne
Cellulose-Specific Type B Carbohydrate Binding Modules: Understanding Oligomeric And Non-Crystalline Substrate Recognition Mechanisms, Abhishek A. Kognole, Christina M. Payne
Chemical and Materials Engineering Faculty Publications
Background: Effective enzymatic degradation of crystalline polysaccharides requires a synergistic cocktail of hydrolytic enzymes tailored to the wide-ranging degree of substrate crystallinity. To accomplish this type of targeted carbohydrate recognition, nature produces multi-modular enzymes, having at least one catalytic domain appended to one or more carbohydrate binding modules (CBMs). The Type B CBM categorization encompasses several families (i.e., protein folds) of CBMs that are generally thought to selectively bind oligomeric polysaccharides; however, a subset of cellulose-specific CBM families (17 and 28) appear to bind non-crystalline cellulose more tightly than oligomers and in a manner that discriminates between surface topology.
Results: …
Correlation Of Structure, Function And Protein Dynamics In Gh7 Cellobiohydrolases From Trichoderma Atroviride, T. Reesei And T. Harzianum, Anna S. Borisova, Elena V. Eneyskaya, Suvamay Jana, Silke F. Badino, Jeppe Kari, Antonella Amore, Magnus Karlsson, Henrik Hansson, Mats Sandgren, Michael E. Himmel, Peter Westh, Christina M. Payne, Anna A. Kulminskaya, Jerry Ståhlberg
Correlation Of Structure, Function And Protein Dynamics In Gh7 Cellobiohydrolases From Trichoderma Atroviride, T. Reesei And T. Harzianum, Anna S. Borisova, Elena V. Eneyskaya, Suvamay Jana, Silke F. Badino, Jeppe Kari, Antonella Amore, Magnus Karlsson, Henrik Hansson, Mats Sandgren, Michael E. Himmel, Peter Westh, Christina M. Payne, Anna A. Kulminskaya, Jerry Ståhlberg
Chemical and Materials Engineering Faculty Publications
Background: The ascomycete fungus Trichoderma reesei is the predominant source of enzymes for industrial conversion of lignocellulose. Its glycoside hydrolase family 7 cellobiohydrolase (GH7 CBH) TreCel7A constitutes nearly half of the enzyme cocktail by weight and is the major workhorse in the cellulose hydrolysis process. The orthologs from Trichoderma atroviride (TatCel7A) and Trichoderma harzianum (ThaCel7A) show high sequence identity with TreCel7A, ~ 80%, and represent naturally evolved combinations of cellulose-binding tunnel-enclosing loop motifs, which have been suggested to influence intrinsic cellobiohydrolase properties, such as endo-initiation, processivity, and off-rate.
Results: The TatCel7A, ThaCel7A, …