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Full-Text Articles in Engineering
Osteogenic Potential Of Poly(Ethylene Glycol)–Poly(Dimethylsiloxane) Hybrid Hydrogels, Dany Munoz Pinto, Andrea Jimenez-Vergara, Yaping Hou, Heather Hayenga, Alejandra Rivas, Melissa Grunlan, Mariah Hahn
Osteogenic Potential Of Poly(Ethylene Glycol)–Poly(Dimethylsiloxane) Hybrid Hydrogels, Dany Munoz Pinto, Andrea Jimenez-Vergara, Yaping Hou, Heather Hayenga, Alejandra Rivas, Melissa Grunlan, Mariah Hahn
Dany J. Munoz Pinto
Growth factors have been shown to be potent mediators of osteogenesis. However, their use in tissue-engineered scaffolds not only can be costly but also can induce undesired responses in surrounding tissues. Thus, the ability to specifically induce osteogenic differentiation in the absence of exogenous growth factors through manipulation of scaffold material properties would be desirable for bone regeneration. Previous research indicates that addition of inorganic or hydrophobic components to organic, hydrophilic scaffolds can enhance multipotent stem cell (MSC) osteogenesis. However, the combined impact of scaffold inorganic content and hydrophobicity on MSC behavior has not been systematically explored, particularly in three-dimensional …
Effect Of Applied Uniaxial Stress On Rate And Mechanical Effects Of Cross-Linking In Tissue-Derived Biomaterials, Debbie Chachra, Paul Gratzer, Christopher Pereira, J. Lee
Effect Of Applied Uniaxial Stress On Rate And Mechanical Effects Of Cross-Linking In Tissue-Derived Biomaterials, Debbie Chachra, Paul Gratzer, Christopher Pereira, J. Lee
Debbie Chachra
Conformational changes in collagen fibrils, and indeed the triple helix, can be produced by application of mechanical stress or strain. We have demonstrated that the rate of cross-linking in glutaraldehyde and epoxide homobifunctional reagents can be modulated by uniaxial stress (strain). Two poly(glycidyl ether) epoxides were used: Denacol® EX-810 (a small bifunctional reagent), and Denacol EX-512 (a large polyfunctional reagent). To prevent any possible effect from being masked by saturation of cross-linking sites, bovine pericardium was cross-linked to such an extent that the increase in collagen denaturation temperature, Td, was one-half of the maximal rise achievable with …
Impact Of Endothelial Cells And Mechanical Conditioning On Smooth Muscle Cell Extracellular Matrix Production And Differentiation, Allen Bulick, Dany Munoz Pinto, Xin Qu, Mousami Mani, Deissy Cristancho, Matthew Urban, Mariah Hahn
Impact Of Endothelial Cells And Mechanical Conditioning On Smooth Muscle Cell Extracellular Matrix Production And Differentiation, Allen Bulick, Dany Munoz Pinto, Xin Qu, Mousami Mani, Deissy Cristancho, Matthew Urban, Mariah Hahn
Dany J. Munoz Pinto
The aim of the current study was to explore the separate and coupled effects of endothelial cell (EC) presence and mechanical conditioning on smooth muscle cell (SMC) responses by combining bilayered poly(ethylene glycol) diacrylate (PEGDA) hydrogels with a pulsatile flow bioreactor. Each construct was composed of an outer PEGDA layer containing SMC and an inner PEGDA layer, either with or without EC. After an initial 3 days of static culture, EC+ and EC− constructs were each further divided into two subgroups, half of which received mechanical conditioning mimetic of late gestation (mean pressures of ∼50 mmHg and peak-to-trough pressure differentials …