Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Engineering
Impact Of Endothelial Cells And Mechanical Conditioning On Smooth Muscle Cell Extracellular Matrix Production And Differentiation, Allen Bulick, Dany Munoz Pinto, Xin Qu, Mousami Mani, Deissy Cristancho, Matthew Urban, Mariah Hahn
Impact Of Endothelial Cells And Mechanical Conditioning On Smooth Muscle Cell Extracellular Matrix Production And Differentiation, Allen Bulick, Dany Munoz Pinto, Xin Qu, Mousami Mani, Deissy Cristancho, Matthew Urban, Mariah Hahn
Dany J. Munoz Pinto
The aim of the current study was to explore the separate and coupled effects of endothelial cell (EC) presence and mechanical conditioning on smooth muscle cell (SMC) responses by combining bilayered poly(ethylene glycol) diacrylate (PEGDA) hydrogels with a pulsatile flow bioreactor. Each construct was composed of an outer PEGDA layer containing SMC and an inner PEGDA layer, either with or without EC. After an initial 3 days of static culture, EC+ and EC− constructs were each further divided into two subgroups, half of which received mechanical conditioning mimetic of late gestation (mean pressures of ∼50 mmHg and peak-to-trough pressure differentials …