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Lamina Propria Cellularity And Collagen Composition: An Integrated Assessment Of Structure In Humans, Dany Munoz Pinto, Peter Whittaker, Mariah Hahn
Lamina Propria Cellularity And Collagen Composition: An Integrated Assessment Of Structure In Humans, Dany Munoz Pinto, Peter Whittaker, Mariah Hahn
Dany J. Munoz Pinto
Objectives: In this study, we quantitatively examined cell density, collagen types I and III, and regional variations in collagen fiber thickness and orientation in the human midmembranous vocal fold lamina propria (LP).
Methods: Lamina propria samples were solubilized with proteinase K or with cyanogen bromide. Cell density was assessed in proteinase K digests by measuring DNA and normalizing it to tissue total protein. Collagen types I and III were quantified by enzyme-linked immunosorbent assay–based detection of collagen type–specific peptides generated by cyanogen bromide digestion. In addition, LP total collagen was determined by measuring sample hydroxyproline levels. Variations in collagen fiber …