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Survey Of The Performance Of Gold Nanoparticles Within An Ssdna-Stabilized Biosensor For The Detection Of Hg2+, Madalyn Zagajowski
Survey Of The Performance Of Gold Nanoparticles Within An Ssdna-Stabilized Biosensor For The Detection Of Hg2+, Madalyn Zagajowski
Pence-Boyce STEM Student Scholarship
Methods
5 nm AuNPs were synthesized by combining solutions of HAuCl4 and sodium citrate, followed by dropwise addition of NaBH4 solution and aging for 24 hours. Additionally, Hg-bound poly(30)T ssDNA oligos were prepared by incubating with HgCl2 solution, with exonuclease I being added to denature unbound DNA. Aliquots of the AuNP solution were then treated with the ssDNA, either Hg-bound or non-Hg bound, and UV-vis and fluorescence spectra were taken of the samples with a Shimadzu UV-1280 spectrophotometer and a Hitachi F-2500 fluorescence spectrophotometer.
Results & Discussion
The UV-vis spectral data of the non-Hg bound AuNP/ssDNA complex samples did not …