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Defining An Optimal Surface Chemistry For Pluripotent Stem Cell Culture In 2d And 3d, Michael Roy Zonca Jr.
Defining An Optimal Surface Chemistry For Pluripotent Stem Cell Culture In 2d And 3d, Michael Roy Zonca Jr.
Legacy Theses & Dissertations (2009 - 2024)
Surface chemistry is critical for growing pluripotent stem cells in an undifferentiated state. There is great potential to engineer the surface chemistry at the nanoscale level to regulate stem cell adhesion. However, the challenge is to identify the optimal surface chemistry of the substrata for ES cell attachment and maintenance. Using a high-throughput polymerization and screening platform, a chemically defined, synthetic polymer grafted coating that supports strong attachment and high expansion capacity of pluripotent stem cells has been discovered using mouse embryonic stem (ES) cells as a model system. This optimal substrate, N-[3-(Dimethylamino)propyl] methacrylamide (DMAPMA) that is grafted on 2D …