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Full-Text Articles in Engineering
Inkjet Printing For High Throughput Cell Patterning, Elisabeth Roth, Tao Xu, Mainak Das, Cassie Gregory, Jay Hickman, Thomas Boland
Inkjet Printing For High Throughput Cell Patterning, Elisabeth Roth, Tao Xu, Mainak Das, Cassie Gregory, Jay Hickman, Thomas Boland
Thomas Boland
The adaptation of inkjet printing technology to the complex fields of tissue engineering and biomaterial development presents the potential to increase progress in these emerging technologies through the implementation of this high-throughput capability via automated processes to enable precise control and repeatability. In this paper, a method of applying high-throughput inkjet printing to control cellular attachment and proliferation by precise, automated deposition of collagen is presented. The results indicate that commercial inkjet printing technology can be used to create viable cellular patterns with a resolution of 350 microm through the deposition of biologically active proteins. This method demonstrates a combination …
Construction Of High- Density Bacterial Colony Arrays And Patterns By The Ink Jet Method, Tao Xu, Sevastioni Petridou, Eric Lee, Elisabeth Roth, Narendra Vyavahare, Jay Hickman, Thomas Boland
Construction Of High- Density Bacterial Colony Arrays And Patterns By The Ink Jet Method, Tao Xu, Sevastioni Petridou, Eric Lee, Elisabeth Roth, Narendra Vyavahare, Jay Hickman, Thomas Boland
Thomas Boland
We have developed a method for fabricating bacterial colony arrays and complex patterns using commercially available ink-jet printers. Bacterial colony arrays with a density of 100 colonies/cm(2) were obtained by directly ejecting Escherichia coli (E. coli) onto agar-coated substrates at a rapid arraying speed of 880 spots per second. Adjusting the concentration of bacterial suspensions allowed single colonies of viable bacteria to be obtained. In addition, complex patterns of viable bacteria as well as bacteria density gradients were constructed using desktop printers controlled by a simple software program.
Tissue-Engineering Constructs, Using Photopolymerizable Hydrogels And Stereolithography.”, Busaina Dhariwala, Elaine Hunt, Thomas Boland
Tissue-Engineering Constructs, Using Photopolymerizable Hydrogels And Stereolithography.”, Busaina Dhariwala, Elaine Hunt, Thomas Boland
Thomas Boland
One of the most important aspects of tissue engineering is the design of the scaffold providing the mechanical strength and access to nutrients for the new tissue. For customized tissue engineering, it is essential to be able to fabricate three-dimensional scaffolds of various geometric shapes, in order to repair defects caused by accidents, surgery, or birth. Rapid prototyping or solid free-form fabrication (SFF) techniques hold great promise for designing three-dimensional customized scaffolds, yet traditional cell-seeding techniques may not provide enough cell mass for larger constructs. This article presents a novel attempt to fabricate three-dimensional scaffolds, using hydrogels combined with cell …