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Biocompatibility Of Poly-Ε-Caprolactone-Hydroxyapatite Composite On Mouse Bone Marrow-Derived Osteoblasts And Endothelial Cells, Haiying Yu, Paul H. Wooley, Shang-You Yang
Biocompatibility Of Poly-Ε-Caprolactone-Hydroxyapatite Composite On Mouse Bone Marrow-Derived Osteoblasts And Endothelial Cells, Haiying Yu, Paul H. Wooley, Shang-You Yang
Wayne State University Associated BioMed Central Scholarship
Abstract
Background
Tissue-engineered bone may be developed by seeding the cells capable of both osteogenesis and vascularization on biocompatible composite scaffolds. The current study investigated the performance of mice bone marrow-derived osteogenic cells and endothelial cells as seeded on hydroxyapatite (HA) and poly-ε-caprolactone (PCL) composite scaffolds.
Methods
Mononuclear cells were induced to osteoblasts and endothelial cells respectively, which were defined by the expression of osteocalcin, alkaline phosphatase (ALP), and deposits of calcium-containing crystal for osteoblasts, or by the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) and von Willebrand factor (vWF), and the formation of a capillary network in Matrigel™ …