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Design And Experimental Validation Of Continuous Dna Separation Using Itp-Based Microfluidic Device, Nora C. M. Goscinski, Mary E. Rosa, Elizabeth K. Wheeler, Maxim Shusteff
Design And Experimental Validation Of Continuous Dna Separation Using Itp-Based Microfluidic Device, Nora C. M. Goscinski, Mary E. Rosa, Elizabeth K. Wheeler, Maxim Shusteff
Chemistry and Biochemistry
We have demonstrated a chip-based microfluidic device suitable for rapid and high-throughput DNA separation and purification for downstream analysis. The glass-based device incorporates a transverse free-flow isotachophoresis (tFF-ITP) that overcomes the volume limitations of CE approaches, and allows continuous sample processing in contrast to batch-mode solid-phase extraction (SPE). The device is able to focus DNA at flow rates up to 100 uL/min, and sample conductivity up to 2 mS/cm. Downstream of the chip, 30-40% of DNA from the input sample is recovered as a result of ITP focusing, and preliminary results indicate that this design is able to purify DNA …