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Atomic Force Microscopy Used To Determine The Young's Modulus Of Vascular Smooth Muscle Cells, Samuel C. Lieber

Theses

The Young's modulus of cultured mouse and rat vascular smooth muscle cells (VSMC) was measured with an atomic force microscope (AFM). The AFM can image the three-dimensional structure of biological cells in a physiological environment enabling real-time biochemical and physiological processes to be monitored at a resolution similar to that obtained for the electron microscope (EM). Cellular mechanical properties were determined by indenting the cell's body, and analyzing the indentation data with classical infinitesimal strain theory. This calculation was accomplished by modeling the AFM probe as a cone. as well as a blunted cone. The blunted cone geometry fits the …