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Development Of Microdialysis Probes In Series Approach Toward Eliminating Microdialysis Sampling Calibration: Miniaturization Into A Pdms Microfluidic Device, Randy Espinal Cabrera
Development Of Microdialysis Probes In Series Approach Toward Eliminating Microdialysis Sampling Calibration: Miniaturization Into A Pdms Microfluidic Device, Randy Espinal Cabrera
Graduate Theses and Dissertations
A new microdialysis sampling method and microfluidic device were developed in vitro. The method consisted of using up to four microdialysis sampling probes connected in series to evaluate the relative recovery (RR) of different model solutes methyl orange, fluorescein isothiocyanate (FITC)-dextran average mol. wt. 4,000 (FITC-4), FITC-10, FITC-20, and FITC-40. Different flow rates (0.8, 1.0, and 1.5 µL/min) were used to compare experimentally observed relative recoveries with theoretical estimations. With increasing the number of probes in series, the relative recovery increases and ~100% (99.7% ± 0.9%) relative recovery for methyl orange was obtained. For larger molecules such as fluorescein isothiocyanate …
Design, Fabrication, And Testing Of A 3d Printer Based Microfluidic System, Carlton A. Mcmullen
Design, Fabrication, And Testing Of A 3d Printer Based Microfluidic System, Carlton A. Mcmullen
Graduate Theses and Dissertations
A pneumatically actuated PDMS based microfluidic devices were designed and fabricated by soft-lithography. Two types of molds were fabricated out of different material for this experiment. The first mold, (device 1), was fabricated from a sheet of Polymethyl methacrylate (PMMA) material, similar to Plexiglas. The device features were micro-engraved onto the face of the material. The second mold, (device 2), was fabricated from the use of fused deposition modeling (FDM) 3D printing. The pumping efficiency of the PDMS devices was analyzed through the characterization of the micro-pumps flowrate with respect to the pumps driving pressure and the actuation frequency. Tested …
Development Of A Pdms Based Micro Total Analysis System For Rapid Biomolecule Detection, Balaji Srinivasan Venkatesh
Development Of A Pdms Based Micro Total Analysis System For Rapid Biomolecule Detection, Balaji Srinivasan Venkatesh
Graduate Theses and Dissertations
The emerging field of micro total analysis system powered by microfluidics is expected to revolutionize miniaturization and automation for point-of-care-testing systems which require quick, efficient and reproducible results. In the present study, a PDMS based micro total analysis system has been developed for rapid, multi-purpose, impedance based detection of biomolecules. The major components of the micro total analysis system include a micropump, micromixer, magnetic separator and interdigitated electrodes for impedance detection. Three designs of pneumatically actuated PDMS based micropumps were fabricated and tested. Based on the performance test results, one of the micropumps was selected for integration. The experimental results …
Factors Affecting Redox Magnetohydrodynamics For Flow In Small Volumes, Matthew D. Gerner
Factors Affecting Redox Magnetohydrodynamics For Flow In Small Volumes, Matthew D. Gerner
Graduate Theses and Dissertations
Lab-on-a-chip technologies offer the possibility of developing analytical devices that are low-cost, portable, disposable, fast, and operable by non-technical personnel. Such devices require automated methods to manipulate ultra-small volumes (picoliters) of samples and solution, including pumping, stirring, and positioning. Current methods for ultra-small volume microfluidics have limitations that restrict their use including high voltage requirements, disadvantageous flow profiles or rates, and relatively complicated fabrication due to mechanical parts. Redox magnetohydrodyanmics (RMHD) that utilizes permanent magnets for portability shows promise as a micropump with ease of switching flow direction, no moving parts, compatibility with both aqueous and non-aqueous solutions, low voltages …