Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Engineering
Optimizing Production Methods For Artifical Silk Proteins Through Bioreactor And Purification Studies Of Recombinant Proteins Expressed From Pichia Pastoris, Aaron Ramey
All Theses
Advancements in the field of biomaterials are being made to produce an artificial silk fiber. A gene was constructed which utilized components from both the dragline silk of Nephila clavipes and nematode collagen. For this engineered protein, the yeast strain Pichia pastoris was chosen to be the host organism. Previous research has shown P. pastoris to have comparatively low amounts of specific protein productivity. Therefore, this problem must be compensated for by obtaining extremely high cell densities. The main focus of this study was to optimize the fermentation parameters of transgenic yeast cultures within a bioreactor in order to increase …
Influence Of Temperature And Polymer/ Mold Surface Interactions On Micro-Feature Replication At Ambient Pressure In Micro Injection Molding, Varun Thakur
All Theses
This study assesses micro-feature replication at elevated mold temperature and ambient pressure using a variety of polymers. Moldings were made on the surface of stainless steel and silicon wafers containing features as small as 130 nm under isothermal conditions in a heated chamber by allowing polymer to spread over the features at ambient pressure. Feature replication was analyzed via AFM and SEM. Polymer behavior was characterized via melt contact angle and melt viscosity. While feature detail was well replicated, the moldings exhibited poor dimensional accuracy due to shrinkage. In general, polymers with low viscosity and surface tension comparable to the …
Dielectric Properties And Method Of Characterizing Ceramic Powders And Multiphase Composites, Ravi Kiran Kota
Dielectric Properties And Method Of Characterizing Ceramic Powders And Multiphase Composites, Ravi Kiran Kota
All Theses
Barium Titanate was the first developed ferroelectric ceramic material and even today it is widely used. It is also a well-known material used for capacitors. The only reason behind a wide range of applications is that barium titanate boasts of high dielectric properties. A phase transition from cubic to tetragonal at normal working temperatures provides enhanced dielectric properties in this electronic material. For any application and design, the most inquired property is the dielectric constant. Predicting the dielectric constant is very much required as it is the pre-requisite. This is the major objective of the present work. To estimate the …