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Production Of Poly-?-Glutamic Acid By Bacillus Licheniformis: Synthesis And Characterization, N. Konglom, C. Chuensangjun, C. Pechyen, S. Sirisansaneeyakul
Production Of Poly-?-Glutamic Acid By Bacillus Licheniformis: Synthesis And Characterization, N. Konglom, C. Chuensangjun, C. Pechyen, S. Sirisansaneeyakul
Journal of Metals, Materials and Minerals
Poly-?-glutamic acid (?-PGA), one type of amino acid polymers, consists of D-and L-glutamic acids linked with amide bonding between ?-amino and ?-carboxylic acids. Naturally, ?-PGA could be biodegradable polymer and water-solubleapplied in various industries such as food, cosmetics, and pharmaceutical industries. Variation of initial L-glutamic acid concentration affecting on the production of ?-PGA by fermentation of Bacillus licheniformis was studied. The batch fermentation process consists of preparing the inoculum and expanding the culture of B. licheniformis into E medium (pH 6.5) for ?-PGA production. ?-PGA was recovered by using methanol precipitation together with lyophilization. For characterization, the molecular size of …