Engineering Commons^™

Effectiveness Of Chain Link Turtle Fence And Culverts In Reducing Turtle Mortality And Providing Connectivity Along U.S. Hwy 83, Valentine National Wildlife Refuge, Nebraska, Usa, Marcel P. Huijser, Kari E. Gunson, Elizabeth R. Fairbank

Nebraska Department of Transportation: Research Reports

We evaluated the effectiveness of existing turtle fences through collecting and analyzing turtle mortality data along U.S. Hwy 83, in and around Valentine National Wildlife Refuge, Nebraska, USA. We also investigated the level of connectivity for turtles provided through the culverts that were originally designed to pass water through a capture-mark-recapture experiment. While fenced valley sections had 33.1% fewer turtle observations than unfenced valley sections, the difference was not significant. However, we think that the effectiveness of the fence can be improved through fence repairs, other modifications of the fences, vegetation maintenance, and extending the length of the fences. Four …

Analytical Modeling Of A Communication Channel Based On Subthreshold Stimulation Of Neurobiological Networks, Alireza Khodaei

Department of Computer Science and Engineering: Dissertations, Theses, and Student Research

The emergence of wearable and implantable machines manufactured artificially or synthesized biologically opens up a new horizon for patient-centered health services such as medical treatment, health monitoring, and rehabilitation with minimized costs and maximized popularity when provided remotely via the Internet. In particular, a swarm of machines at the scale of a single cell down to the nanoscale can be deployed in the body by the non-invasive or minimally invasive operation (e.g., swallowing and injection respectively) to perform various tasks. However, an individual machine is only able to perform basic tasks so it needs to exchange data with the others …

Individual And Community Well-Being In Nonmetropolitan Nebraska: 2017 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L. J. Mcelravy, T. L. Meyer

Rural Futures Institute: Publications

Conclusion

By many different measures, rural Nebraskans are positive about their community. Many rural Nebraskans rate their community favorably on its social dimensions: as friendly, trusting and supportive. Most rural Nebraskans say it would be difficult to leave their community and have a positive attachment to their community. And, most rural Nebraskans disagree that their community is powerless to control its future.

Across all years of this study, rural Nebraskans’ views about the change in their community have generally been positive. The proportion believing their community has changed for the better during the past year has usually been greater than …

Effects Of Nitrogen And Phosphorus Fertilizer And Topsoil Amendment On Native Plant Cover In Roadside Revegetation Projects, Heidi L. Hillhouse, Walter H. Schacht, Jonathan M. Soper, Carol E. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Establishing vegetation on roadsides following construction can be challenging, especially for relatively slow growing native species. Topsoil is generally removed during construction, and the surface soil following construction (“cut-slope soils”) is often compacted and low in nutrients, providing poor growing conditions for vegetation. Nebraska Department of Transportation (NDOT) protocols have historically called for nitrogen (N) and phosphorus (P) fertilization when planting roadside vegetation following construction, but these recommendations were developed for cool-season grass plantings and most current plantings use slower-establishing, native warmseason grasses that may benefit less than expected from current planting protocols. We evaluated the effects of nitrogen and …

Cross-Species Complementation Reveals Conserved Functions For Early Flowering 3 Between Monocots And Dicots, He Huang, Malia A. Gehan, Sarah E. Huss, Sohpie Alvarez, Cesar Lizarraga, Ellen L. Gruebbling, John Gierer, Michael J. Naldrett, Rebacca K. Bindbeutel, Bradley S. Evans, Todd C. Mockler, Dmitri A. Nusinow

Nebraska Center for Biotechnology: Faculty and Staff Publications

Plant responses to the environment are shaped by external stimuli and internal signaling pathways. In both the model plant Arabidopsis thaliana (Arabidopsis) and crop species, circadian clock factors are critical for growth, flowering, and circadian rhythms. Outside of Arabidopsis, however, little is known about the molecular function of clock gene products. Therefore, we sought to compare the function of Brachypodium distachyon (Brachypodium) and Setaria viridis (Setaria) orthologs of EARLY FLOWERING 3, a key clock gene in Arabidopsis. To identify both cycling genes and putative ELF3 functional orthologs in Setaria, a …

Media, Institutions And Voting: Perceptions Of Nonmetropolitan Nebraskans: 2017 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L. J. Mcelravy, Timothy L. Meyer

Rural Futures Institute: Publications

Conclusion

Rural Nebraskans most trust information received from local news sources (TV and newspapers) and public sources (PBS and public radio). They least trust information from social networking sites and Internet blogs. Most rural Nebraskans are somewhat or very confident in their ability to recognize news that is made up.

Most rural Nebraskans have confidence in their local institutions (public safety agencies in their community; public schools in their community; and voting and election systems in their county). On the other hand, over one-quarter of rural Nebraskans have very little confidence in the following national institutions: U.S. House of Representatives, …

Improving Wildflower Longevity In Roadside Seeding Areas, Walter Schacht, Jonathan M. Soper, Carol Wienhold

Nebraska Department of Transportation: Research Reports

Re-vegetation efforts on bare roadsides of newly-constructed highways are primarily focused on the stabilization of soil to reduce rates of erosion. The Nebraska Department of Transportation (NDOT) seeds roadsides with a diverse mixture of grasses and wildflowers for site stabilization as well as to enhance the visual quality of roadsides. Although grasses dominate roadside plantings in terms of cover and density, wildflowers are largely responsible for the visual enhancement of recently-seeded roadsides. In addition to the visual component, wildflowers provide essential ecological functions on roadsides. Wildflowers improve water and nutrient cycling in the compacted roadside soils by increasing water infiltration …

Shopping For Food In Nonmetropolitan Nebraska: 2017 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L.J. Mcelravy, Timothy L. Meyer, Vanessa Wielenga

Rural Futures Institute: Publications

Conclusion

Rural Nebraskans purchase their food from a variety of stores. Almost four in ten buy most of their food from a supercenter (like Wal-Mart or Costco). Just over three in ten typically shop at a supermarket and just under three in ten buy most of their food at a small grocery store. Differences in the type of store most utilized occur by community size. Persons living in or near larger communities are more likely than persons living in or near smaller communities to purchase the majority of their food from either a supermarket or supercenter.

Persons living in or …

Perceptions Of Agriculture And The Economy In Nonmetropolitan Nebraska: 2017 Nebraska Rural Poll Results, Rebecca J. Vogt, Cheryl A. Burkhart-Kriesel, Randolph L. Cantrell, Bradley Lubben, L.J. Mcelravy, Timothy L. Meyer

Rural Futures Institute: Publications

Conclusion

Most rural Nebraskans view their economic well-being as being dependent on agriculture. Over three-fourths feel their economic well-being is at least somewhat tied to agriculture. Economic dependence on agriculture has changed very little over the past seven years. The proportions reported in 2017 are almost identical to those from 2010.

Similarly, most rural Nebraskans believe the economic well-being of their community is very much dependent on agriculture. Overall, over nine in ten rural Nebraskans feel their community’s economic well-being is at least somewhat tied to agriculture.

Most rural Nebraskans say their job/income security is about the same as it …

Cross-Resistance And Synergism Bioassays Suggest Multiple Mechanisms Of Pyrethroid Resistance In Western Corn Rootworm Populations, Adriano E. Pereira, Dariane Souza, Sarah N. Zukoff, Lance J. Meinke, Blair D. Siegfried

Department of Entomology: Faculty Publications

Recently, resistance to the pyrethroid bifenthrin was detected and confirmed in field populations of western corn rootworm, Diabrotica virgifera virgifera LeConte from southwestern areas of Nebraska and Kansas. As a first step to understand potential mechanisms of resistance, the objectives of this study were i) to assess adult mortality at diagnostic concentration- LC99 to the pyrethroids bifenthrin and tefluthrin as well as DDT, ii) estimate adult and larval susceptibility to the same compounds as well as the organophosphate methyl-parathion, and iii) perform synergism experiments with piperonyl butoxide (PBO) (P450 inhibitor) and S,S,S-tributyl-phosphorotrithioate (DEF) (esterase inhibitor) in field populations. Most of …

Geometry-Based Mass Grading Of Mango Fruits Using Image Processing, M. A. Momin, Md Towfiqur Rahman, M. S. Sultana, C. Igathinathane, A. T. M. Ziauddin, T. E. Grift

Department of Biological Systems Engineering: Papers and Publications

Mango (Mangifera indica) is an important, and popular fruit in Bangladesh. However, the post-harvest processing of it is still mostly performed manually, a situation far from satisfactory, in terms of accuracy and throughput. To automate the grading of mangos (geometry and shape), we developed an image acquisition and processing system to extract projected area, perimeter, and roundness features. In this system, images were acquired using a XGA format color camera of 8-bit gray levels using fluorescent lighting. An image processing algorithm based on region based global thresholding color binarization, combined with median filter and morphological analysis was developed …

Genetic Code Expansion In Biochemical Investigations And Biomedical Applications, Nanxi Wang

Department of Chemistry: Dissertations, Theses, and Student Research

Genetic code expansion provides a powerful tool for site-specific incorporation of unnatural amino acids (unAAs) with novel biochemical and physiological properties into proteins in live cells and organisms. To achieve this, a nonsense codon suppression system, which consists of an orthogonal aminoacyl-tRNA synthetase (aaRS) and tRNA pair that specifically decodes a nonsense codon (e.g., amber codon and quadruplet codon) with an unAA but do not “cross talk” with their endogenous counterparts, was established. This Ph.D. thesis presents our efforts on evolution and application of nonsense codon suppression systems for biochemical and biomedical investigations.

In Chapter 1, a brief overview of …

High Throughput In Vivo Analysis Of Plant Leaf Chemical Properties Using Hyperspectral Imaging, Piyush Pandey, Yufeng Ge, Vincent Stoerger, James C. Schnable

Department of Biological Systems Engineering: Dissertations, Theses, and Student Research

The possibility of predicting plant leaf chemical properties using hyperspectral images was studied. Sixty maize and 60 soybean plants were used, and two experiments were conducted: one with water limitation and the second with nutrient limitation, with the purpose of creating wide ranges of these chemical properties in plant leaf tissues. A hyperspectral imaging system with a spectral range from 550 to 1700 nm was used to acquire plant images in a high throughput fashion (plants placed on an automated conveyor belt). Leaf chemical properties were measured in the laboratory. Partial least squares regression was implemented on spectral data to …

Performance Of Seed Treatments And In-Furrow At-Plant Insecticides For Protection Against Cry3bb1-Resistant Western Corn Rootworm, 2015, Jonas Victor De Macedo,, Kayla A. Mollet, Julie A. Peterson

Department of Entomology: Faculty Publications

The western corn rootworm is an important pest of corn that can compromise yield by feeding on plant roots during its larval stage. WCRW management has been complicated by the development of resistance in some regions, including Nebraska, to transgenic Bacillus thuringiensis (Bt) traits, particularly the protein Cry3Bb1, which confers cross-resistance to mCry3A. A field trial was established to evaluate the efficacy of neonicotinoid seed treatments in combination with in-furrow insecticides on a corn hybrid expressing mCry3A Bt proteins against corn rootworm in an area with a history of rootworm resistance. The trial was conducted in a farmer’s field in …

Rainbow Peacock Spiders Inspire Miniature Superiridescent Optics, Bor-Kai Hsiung, Radwanul Hasan Siddique, Doekele G. Stavenga, Jürgen C. Otto, Michael C. Allen, Ying Liu, Yongfeng Lu, Dimitri D. Deheyn, Matthew D. Shawkey, Todd A. Blackledge

Department of Electrical and Computer Engineering: Faculty Publications

Colour produced by wavelength-dependent light scattering is a key component of visual communication in nature and acts particularly strongly in visual signalling by structurallycoloured animals during courtship. Two miniature peacock spiders (Maratus robinsoni and M. chrysomelas) court females using tiny structured scales (~ 40 × 10 μm²) that reflect the full visual spectrum. Using TEM and optical modelling, we show that the spiders’ scales have 2D nanogratings on microscale 3D convex surfaces with at least twice the resolving power of a conventional 2D diffraction grating of the same period. Whereas the long optical path lengths required …

Evolutionary History Of Chemosensory-Related Gene Families Across The Arthropoda, Seong-Il Eyun, Ho Young Soh, Marijan Posavi, James B. Munro, Daniel S.T. Hughes, Shwetha C. Murali, Jiaxin Qu, Shannon Dugan, Sandra L. Lee, Hsu Chao, Huyen Dinh, Yi Han, Harshavardhan Doddapaneni, Kim C. Worley, Donna M. Muzny, Eun-Ok Park, Joana C. Silva, Richard A. Gibbs, Stephen Richards, Carol Eunmi Lee

Nebraska Center for Biotechnology: Faculty and Staff Publications

Chemosensory-related gene (CRG) families have been studied extensively in insects, but their evolutionary history across the Arthropoda had remained relatively unexplored. Here, we address current hypotheses and prior conclusions on CRG family evolution using a more comprehensive data set. In particular, odorant receptors were hypothesized to have proliferated during terrestrial colonization by insects (hexapods), but their association with other pancrustacean clades and with independent terrestrial colonizations in other arthropod subphyla have been unclear. We also examine hypotheses on which arthropod CRG family is most ancient. Thus, we reconstructed phylogenies of CRGs, including those from new arthropod genomes and transcriptomes, and …

Biosimp: Using Software Testing Techniques For Sampling And Inference In Biological Organisms, Mikaela Cashman, Jennie L. Catlett, Myra B. Cohen, Nicole R. Buan, Zahmeeth Sakkaff, Massimiliano Pierobon, Christine A. Kelley

CSE Conference and Workshop Papers

Years of research in software engineering have given us novel ways to reason about, test, and predict the behavior of complex software systems that contain hundreds of thousands of lines of code. Many of these techniques have been inspired by nature such as genetic algorithms, swarm intelligence, and ant colony optimization. In this paper we reverse the direction and present BioSIMP, a process that models and predicts the behavior of biological organisms to aid in the emerging field of systems biology. It utilizes techniques from testing and modeling of highly-configurable software systems. Using both experimental and simulation data we show …

Effects Of Post Eviction Resettlement On Land-Use And Cover Change In Uganda’S Oil Exploration Areas, Joseph Ssekandi, John Mburu, Oliver Wasonga, Laban Macopiyo, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Evaluation of the changes in land use and land cover change (LULCC) in respect to oil exploration across the Albertine region in Uganda has been focused around the exploration areas and protected areas, with no attention to the potential impacts of evictees’ activities on resettled areas. This study used LANDSAT images to analyze the land use and land cover changes (LULCC) among the period before eviction (2002 and 2005) at the climax of eviction and resettlements (2005-2011), and during the post-resettlement period (2011-2015) to quantify the impacts of resettlements on the environment. LANDSAT images were processed using ERDAS IMAGINE software …

A Method For Measuring The Attachment Strength Of The Cestode Hymenolepis Diminuta To The Rat Intestine, Wanchuan Xie, Gábor R. Rácz, Benjamin S. Terry, Scott Lyell Gardner

Scott L. Gardner Publications

A unique adaptation of many internal parasites of mammals is their ability to stay in the intestine for extended periods of time and resist the normal peristaltic movements and forces that push and expel material. To better understand parasite adhesion behavior and replicate their attachment method in medical devices, an experiment was designed and performed using the rat tapeworm, Hymenolepis diminuta. The experiment employed a tensile test machine and a digital scale and was designed to calculate the attachment strength of the scolex to the mucosa through the change of the value of the digital scale during the tensile …

Phylogenomic Analysis Of Copepoda (Arthropoda, Crustacea) Reveals Unexpected Similarities With Earlier Proposed Morphological Phylogenies, Seong-Il Eyun

Nebraska Center for Biotechnology: Faculty and Staff Publications

Background: Copepods play a critical role in marine ecosystems but have been poorly investigated in phylogenetic studies. Morphological evidence supports the monophyly of copepods, whereas interordinal relationships continue to be debated. In particular, the phylogenetic position of the order Harpacticoida is still ambiguous and inconsistent among studies. Until now, a small number of molecular studies have been done using only a limited number or even partial genes and thus there is so far no consensus at the order-level.

Results: This study attempted to resolve phylogenetic relationships among and within four major copepod orders including Harpacticoida and the phylogenetic position of …