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Proteomic Analysis Of Ethene-Enriched Groundwater Microcosms From A Vinyl Chloride-Contaminated Site, Adina S. Chuang, Yang Oh Jin, Laura S. Schmidt, Yalan Li, Samuel Fogel, Donna Smoler, Timothy E. Mattes
Proteomic Analysis Of Ethene-Enriched Groundwater Microcosms From A Vinyl Chloride-Contaminated Site, Adina S. Chuang, Yang Oh Jin, Laura S. Schmidt, Yalan Li, Samuel Fogel, Donna Smoler, Timothy E. Mattes
Adina Howe
Contamination of groundwater with vinyl chloride (VC), a known human carcinogen, is a common environmental problem at plastics manufacturing, dry cleaning, and military sites. At many sites, there is the potential to cleanup VC groundwater plumes with aerobic VC-oxidizing microorganisms (e.g., methanotrophs, etheneotrophs, and VC-assimilating bacteria). Environmental biotechnologies that reveal the presence and activity of VC-oxidizing bacteria in contaminated groundwater samples would provide valuable lines of evidence that bioremediation of VC is occurring at a site. We applied targeted shotgun mass spectrometry-based proteomic methods to ethene-enriched groundwater microcosms from a VC-contaminated site. Polypeptides from the enzymes alkene monooxygenase (EtnC) and …
Identification Of Polypeptides Expressed In Response To Vinyl Chloride, Ethene, And Epoxyethane In Nocardioides Sp. Strain Js614 By Using Peptide Mass Fingerprinting, Adina S. Chuang, Timothy E. Mattes
Identification Of Polypeptides Expressed In Response To Vinyl Chloride, Ethene, And Epoxyethane In Nocardioides Sp. Strain Js614 By Using Peptide Mass Fingerprinting, Adina S. Chuang, Timothy E. Mattes
Adina Howe
Enzymes expressed in response to vinyl chloride, ethene, and epoxyethane by Nocardioides sp. strain JS614 were identified by using a peptide mass fingerprinting (PMF) approach. PMF provided insight concerning vinyl chloride biodegradation in strain JS614 and extends the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry as a tool to enhance characterization of biodegradation pathways.