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Electrical and Computer Engineering
Washington University in St. Louis
Electrical & Systems Engineering Publications and Presentations
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A Robust Statistical Estimation (Rose) Algorithm Jointly Recovers The 3d Location And Intensity Of Single Molecules Accurately And Precisely, Hesam Mazidi, Arye Nehorai, Matthew D. Lew
A Robust Statistical Estimation (Rose) Algorithm Jointly Recovers The 3d Location And Intensity Of Single Molecules Accurately And Precisely, Hesam Mazidi, Arye Nehorai, Matthew D. Lew
Electrical & Systems Engineering Publications and Presentations
In single-molecule (SM) super-resolution microscopy, the complexity of a biological structure, high molecular density, and a low signal-to-background ratio (SBR) may lead to imaging artifacts without a robust localization algorithm. Moreover, engineered point spread functions (PSFs) for 3D imaging pose difficulties due to their intricate features. We develop a Robust Statistical Estimation algorithm, called RoSE, that enables joint estimation of the 3D location and photon counts of SMs accurately and precisely using various PSFs under conditions of high molecular density and low SBR.