Engineering Commons^™

Transverse Isotachophoresis Using Polyacrylamide Gel Electrodes, Mark P. Stambaugh

Master's Theses

Separation and isolation of a desired analyte from an impure sample solution containing numerous unwanted interfering agents is the first step of nearly every laboratory test performed in medicine and biology. Nucleic acids are often of particular interest to doctors and researchers, and although methods currently exist for their isolation, these procedures are costly in time, man-power, and real-estate. In addition to easing the execution of presently performed tests, mitigation or elimination of these drawbacks would make a large range of currently unperformed tests both practical and feasible.

This thesis presents a microfluidics-based approach to the isolation of nucleic acids …