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Full-Text Articles in Engineering
Engineering Education Collaboration: Innovative Pedagogical Methods For High School And University Environmentalists, Leslie Hadaway, Megan Urbaitis, Regina Lamendella, Daniel B. Oerther, Andrea C. Burrows, Mike Borowczak, Anant R. Kukreti
Engineering Education Collaboration: Innovative Pedagogical Methods For High School And University Environmentalists, Leslie Hadaway, Megan Urbaitis, Regina Lamendella, Daniel B. Oerther, Andrea C. Burrows, Mike Borowczak, Anant R. Kukreti
Civil, Architectural and Environmental Engineering Faculty Research & Creative Works
This paper presents an innovative teaching approach, how it is implemented, student response results of the implementation, and the assessment of impact on student learning. The findings are based on surveys given to the students after each lab lesson taught in partnership with university (Project STEP) and community members. The purpose of this paper is to showcase authentic molecular technology research methods that have been incorporated into a high school level water quality study in cooperation with a watershed restoration program. Typically, water quality studies focus on chemical analysis such as pH, dissolved oxygen, biochemical oxygen demand, orthophosphates, nitrates, temperature, …
Reverse Transcription Of 16s Rrna To Monitor Ribosome-Synthesizing Bacterial Populations In The Environment, Ting Lu, Peter George Stroot, Daniel B. Oerther
Reverse Transcription Of 16s Rrna To Monitor Ribosome-Synthesizing Bacterial Populations In The Environment, Ting Lu, Peter George Stroot, Daniel B. Oerther
Civil, Architectural and Environmental Engineering Faculty Research & Creative Works
Identification and quantification of phylogenetically defined bacterial populations in the environment are often performed using molecular tools targeting 16S rRNA. Fluorescence in situ hybridization has been used to monitor the expression and processing of rRNA by targeting the 3' tail of precursor 16S rRNA. To expand this approach, we employed reverse transcription of total RNA using primer S-D-Bact-0338-a-A-18. Length heterogeneity detected by slab gel analysis, denaturing high-performance liquid chromatography (DHPLC) was used to differentiate the 5' tail of the precursor from mature 16S rRNA, and the relative abundance of the precursor compared to the abundance of mature 16S rRNA was …