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Civil and Environmental Engineering

Louisiana State University

Theses/Dissertations

Anaerobic

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Full-Text Articles in Engineering

Degradation And Inhibition Of Chlorinated Ethanes And Ethenes In A Two Bed, Sequential Bioremediation System, Alex Boudreau Jan 2013

Degradation And Inhibition Of Chlorinated Ethanes And Ethenes In A Two Bed, Sequential Bioremediation System, Alex Boudreau

LSU Master's Theses

A continuous-flow system composed of two anaerobic bioreactor (ABR) columns (based on engineered wetland systems) connected in series was tested to observe the ability of such a system to treat multiple volatile organic compounds (VOCs) possessing suspected inhibitory effects on the dechlorination of other compounds with cis-1,2-dichloroethylene (cDCE) and 1,1-dichloroethane (1,1-DCA) as the main test chemicals. Following the establishment of cDCE and 1,1-DCA dechlorinating ability at baseline conditions, system perturbations of breakthrough and starvation were conducted to measure the response and recovery of the system dechlorination. In all column experiments, it was observed cDCE dechlorination inhibited the dechlorination of 1,1-DCA …


Reductive Dechlorination Of High Concentrations Of 1,2-Dichloroethane, 1,2-Dichloropropane, And 1,1,2-Trichloroethane By Dehalogenimonas Sp., Andrew D. Maness Jan 2012

Reductive Dechlorination Of High Concentrations Of 1,2-Dichloroethane, 1,2-Dichloropropane, And 1,1,2-Trichloroethane By Dehalogenimonas Sp., Andrew D. Maness

LSU Master's Theses

The research goals presented here were to evaluate the solvent concentrations over which Dehalogenimonas lykanthroporepellens strain BL-DC-9T and Dehalogenimonas sp. IP3-3 are able to reductively dechlorinate 1,2-dichloroethane (1,2-DCA), 1,2-dichloropropane (1,2-DCP), and 1,1,2-trichloroethane (1,1,2-TCA). Both of these novel bacteria were isolated from contaminated groundwater at the PetroProcessors of Louisiana, Inc. Superfund site located near Baton Rouge. Both cluster in the phylum Chloroflexi, related to, but distinct from, the widely studied, reductively dechlorinating “Dehalococcoides” strains .

Although previous research demonstrated that strains BL-DC-9T and IP3-3 are able to reductively dechlorinate a variety of polyhalogenated alkanes, the solvent concentration at which the strains …


Redutive Dehalogenation Of Chlorinated Alkanes By Novel Bacteria At The Petroprocessor Of Louisiana Inc. Superfund Site, Jun Yan Jan 2008

Redutive Dehalogenation Of Chlorinated Alkanes By Novel Bacteria At The Petroprocessor Of Louisiana Inc. Superfund Site, Jun Yan

LSU Doctoral Dissertations

A reductively dehalogenating enrichment culture was established using chloroalkane-contaminated groundwater from the PetroProcessors of Louisiana, Inc. (PPI) Superfund site. Two novel, strictly anaerobic bacterial strains, designated as BL-DC-8 and BL-DC-9, were isolated from the enrichment culture. These strains represent the first bacteria known to anaerobically dehalogenate 1,2,3-trichloropropane, the degradation pathway of which was determined. Both strains could be cultured in completely defined basal medium and were also able to dehalogenate a variety of other vicinally chlorinated alkanes including 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane, 1,2-dichloroethane, and 1,2-dichloropropane via dichloroelimination reactions. Chlorinated alkanes containing only a single chlorine substituent (1-chloropropane, 2-chloropropane), chlorinated alkenes (tetrachloroethene, trichloroethene, …