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The Modeling And Analysis Of Multi-Component, Multivalent Biospecific Adsorption, Bo Henrik Arve

Doctoral Dissertations

"A general model is presented and used to predict the dynamic behavior of the adsorption, wash, and elution stages of biospecific adsorption (affinity chromatography) in finite bath and column operations. The model accounts for film and pore diffusional mass transfer resistances and the rates of interaction between adsorbates and ligands. For column operations the effect of axial dispersion in the flowing fluid stream is included. The model is applicable to single and multi-component biospecific as well as non-specific adsorption, and the adsorbates may be monovalent or multivalent. In the elution stage both non-selective and selective elution of monovalent adsorbates is …