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Articles 1 - 6 of 6
Full-Text Articles in Engineering
The Structure And Stability Of Alpha-Helical, Orthogonal-Bundle Proteins On Surfaces, Shuai Wei
The Structure And Stability Of Alpha-Helical, Orthogonal-Bundle Proteins On Surfaces, Shuai Wei
Theses and Dissertations
The interaction of proteins with surfaces is a major problem involved in protein microarrays. Understanding protein/surface interactions is key to improving the performance of protein microarrays, but current understanding of the behavior of proteins on surfaces is lacking. Prevailing theories on the subject, which suggest that proteins should be stabilized when tethered to surfaces, do not explain the experimentally observed fact that proteins are often denatured on surfaces. In an attempt to develop some predictive capabilities with respect to protein/surface interactions, it was asked in previous works if the stabilization/destabilization of proteins on surfaces could be correlated to secondary structure …
Flammability Limits, Flash Points, And Their Consanguinity: Critical Analysis, Experimental Exploration, And Prediction, Jeffrey R. Rowley
Flammability Limits, Flash Points, And Their Consanguinity: Critical Analysis, Experimental Exploration, And Prediction, Jeffrey R. Rowley
Theses and Dissertations
Accurate flash point and flammability limit data are needed to design safe chemical processes. Unfortunately, improper data storage and reporting policies that disregard the temperature dependence of the flammability limit and the fundamental relationship between the flash point and the lower flammability limit have resulted in compilations filled with erroneous values. To establish a database of consistent flammability data, critical analysis of reported data, experimental investigation of the temperature dependence of the lower flammability limit, and theoretical and empirical exploration of the relationship between flash points and temperature limits are undertaken. Lower flammability limit measurements in a 12-L ASHRAE style …
Syngas Fermentation: Quantification Of Assay Techniques, Reaction Kinetics, And Pressure Dependencies Of The Clostridial P11 Hydrogenase, Bradley E. Skidmore
Syngas Fermentation: Quantification Of Assay Techniques, Reaction Kinetics, And Pressure Dependencies Of The Clostridial P11 Hydrogenase, Bradley E. Skidmore
Theses and Dissertations
Ethanol usage as a transportation fuel is rapidly increasing in the United States. Production of ethanol from cellulose feedstocks via gasification followed by syngas fermentation offers an environmentally friendly approach that mitigates many of the adverse effects associated with production from corn. In the syngas fermentation process, the hydrogenase enzyme of the fermentation bacterium, Clostridium P11 for this work, supplies electrons to the metabolic pathway, facilitating ethanol production. In this thesis, an assay for P11 hydrogenase activity was developed. It was determined that 1) less than 4 minutes of sparging with 50 sccm H2 is needed to reduce O2 levels …
Optimization Of Environmental Conditions To Maximize Carbon Dioxide Sequestration Through Algal Growth, Kenneth M. Karcher
Optimization Of Environmental Conditions To Maximize Carbon Dioxide Sequestration Through Algal Growth, Kenneth M. Karcher
Theses and Dissertations
The micro-alga Chlorella vulgaris was cultivated under a variety of environmental conditions in various culture media solutions to assess and optimize growth rate and biomass productivity. Efforts during this work investigated growth parameters at the micro-scale in an air-lift bubble system with the goal of interpreting performance characteristics that can be applied to a larger serpentine tubular Photo-bioreactor. Maximum growth rates and biomass yields were 0.65 d-1 and 2.003 g biomass/L and achieved in seven days using urea in de-ionized water under a 24:0 Photoperiod (Light:Dark). Additionally, growth rates and biomass yields of 0.65 d-1 and 1.964 g …
Nitrogen And Phosphorus Biomass-Kinetic Model For Chlorella Vulgaris In A Biofuel Production Scheme, William M. Rowley
Nitrogen And Phosphorus Biomass-Kinetic Model For Chlorella Vulgaris In A Biofuel Production Scheme, William M. Rowley
Theses and Dissertations
Chlorella vulgaris was cultured in microbioreactors using Bold’s Basal medium at varying nitrogen and phosphorus concentrations to define nitrogen and phosphorus utilization standards. Nutrient concentration was varied between 137 mg/L to 7.33 mg/L NO3-N and between 55.2 mg/L to11.0 mg/L PO4-P in five test scenarios. All were grown under a constant photoperiod at 22±2 °C and a mixture of 4 to 10% carbon dioxide/air. Maximum yield and growth rate occurred with the highest initial nitrogen and phosphorus concentrations. A statistically significant difference in biomass was found among all test levels at the end of the eight day growth period. Applying …
Extracellular Matrix Based Substrates For Propagation Of Human Pluripotent Stem Cells, Sheena Abraham
Extracellular Matrix Based Substrates For Propagation Of Human Pluripotent Stem Cells, Sheena Abraham
Theses and Dissertations
In human pluripotent stem cell (hPSC) research and applications, the need for a culture system devoid of non-human components is crucial. Such a system should exhibit characteristics observed in conventional culture systems that have used mouse embryonic fibroblast feeders for hPSC self renewal without the requirement of excessive supplementation with growth factors. To achieve this, we focused on the identification and characterization of extracellular matrix (ECM) substrates for hPSC propagation. ECM substrates derived from mouse and human fibroblasts were assessed for their ability to support self-renewal of hPSCs. Characterization of hPSCs on ECM-based substrates demonstrated maintenance of pluripotent characteristics based …