Engineering Commons^™

Optimizing The Release And Methylation Of Bacterial Endospore Dipicolinic Acid, Aaron N. Nackos

Theses and Dissertations

Rapid, portable detection of biological threat agents such as Bacillus anthracis endospores (“spores”) is extremely important given the real and perceived threats of bioterrorism. Gas chromatography-mass spectrometry (GC-MS) is an excellent general means for chemical detection, although special sample preparation and specialized equipment are required to employ GC-MS for detecting biological agents such as spores in the field. A GC sample introduction probe consisting of a helical wire that can be retracted inside a syringe needle, called a coiled wire filament (CWF), was employed as a simple, passively-heated means to introduce the mixture of spores plus reagents for thermochemolysis methylation …