Engineering Commons^™

Mathematical Model Of A Continuous Sterilization System For Fermentation Media Containing Suspended Solids, Mark A. Leskowicz

Theses

A mathematical model has been developed for a continuous sterilization system where the role of suspended solids on the overall degree of sterilization achieved is taken into account. Evaluation of a "typical" commercial continuous sterilization system showed the role of suspended solids to have little effect on the overall sterilization obtained. For continuous sterilization systems operating in a total residence time of less than one minute, the role of solids may become important and should be evaluated.

The Catabolism Of Arginine By Lactobacillus Brevis In A Chemostat, Jeanette Carque

Theses

A total of thirty chemostat runs were evaluated for steady state biomass concentration of L. brevis and arginine deiminase activity at dilution rates ranging from 0.022 to 0.1.07 h^-1 for a substrate feed consisting of 1.0% glucose and 0.5% arginine (w/v). Biomass concentration and enzyme activity responses were also evaluated for variations in glucose concentration from 0.05% to 1.5% and variations in arginine concentrations from 0.25% to 1.0% at a constant dilution rate (0.06 h^-1).

The system exhibited multiple steady states for the range of dilution rates from 0.022 to 0.107 h^-1. A steady state …

The Characterization Of The Oxygen Transfer Capabilities Of Fermentors, Joseph Stephen Adamca

Theses

A method of characterizing the oxygen mass transfer capabilities of fermentors was developed where volumetric oxygen mass transfer coefficients are obtained using a modified sulfite oxidation method in conjunction with on line digital data processing. Carboxymethylcellulose is used in the reaction media to simulate the viscosity of typical non-Newtonian fermentation broths. Using this method, instantaneous values of volumetric oxygen mass transfer coefficients can be obtained for various combinations of agitation and airflow rates in different fermentor configurations. This data is useful in the scale up or scale down of fermentors.

Data obtained using this method have revealed that for a …

A Study On The Growth And Asparaginase Production Of Vibrio Succinogenes In A Chemostat, Preston David Krautheim

Theses

A continuous fermentation assembly was constructed and studies on the growth of the organism, Vibrio succinogenes, and its production of asparaginase was conducted. Results were compared with that already performed on a batch system and showed that a continuous fermentation produced 31.83 I.U. enzymes/mM ammonium formate compared to 17.4 I.U. enzyme/mM formate for batch.

Some of the runs performed indicated that the organism was washing out or giving very low specific growth rates. It was decided that this was not due to operational parameters of the chemostat, but possible chemical contamination in the medium. With all things considered, a continuous …