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Chemical Engineering

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Bioreactors

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Full-Text Articles in Engineering

Mineralization Of 2-Chlorophenol Using A Hollow Fiber Ultrafiltration System In Combination With A Reactor Containing An Immobilized White Rot Fungus, Sheau-Ming Tung Oct 1992

Mineralization Of 2-Chlorophenol Using A Hollow Fiber Ultrafiltration System In Combination With A Reactor Containing An Immobilized White Rot Fungus, Sheau-Ming Tung

Theses

Phanerochaete chrysosporium, a white rot fungus was immobilized on a packed-bed reactor, and was used to mineralize an aqueous stream contaminated with 50 ppm of 2-chlorophenol. Hollow fiber ultrafiltration membranes (for 5,000 daltons and 10,000 daltons molecular weight cut-oft) were utilized to retain the extracellular enzyme resulting from the immobilized fungus in a recirculation loop outside of the reactor.

It was found here that the concentration of proteins (roughly proportional to the extracellular enzyme releasd by the fungus) does not correlate with the rate of degradation of 2-chlorophenol in the system. The protein was concentrated 1.5 fold in the recycle …


Biodegradation Of Phenol And Benzoic Acid In Batch And Sequencing Batch Reactors, Stephane Reynaud Jan 1992

Biodegradation Of Phenol And Benzoic Acid In Batch And Sequencing Batch Reactors, Stephane Reynaud

Theses

A pure culture of Pseudomonas aeruginosa ( ATCC 10145 ) was used for the biodegradation of phenol and benzoic acid. Two sets of small scale (shaker flask) experiments were originally performed: in the first set, phenol was the only carbon source present while in the second, benzoic acid was the sole carbon source. These experiments revealed the kinetics of benzoic acid degradation (they were found to be described by a Monod, non-inhibitory model), as well as of phenol biodegradation (they were found to be described by Andrews'inhibitory model) by Pseudomonas aeruginosa.

The kinetic expressions from the small scale experiments …


Biodegradation Of Phenol And 4-Chlorophenol Using A Single Species In A Sequencing Batch Reactor, Kung-Wei Wang Oct 1991

Biodegradation Of Phenol And 4-Chlorophenol Using A Single Species In A Sequencing Batch Reactor, Kung-Wei Wang

Theses

Biodegradation of phenol and 4-chlorophenol (4CP) using Pseudomonas putida (ATCC 17514) was studied in batch and sequencing batch reactors. Batch experiments were first performed in order to reveal the kinetics of biodegradation for each substrate. 4-Chlorophenol was degradable only in the presence of phenol, or immediately after exposure of the organism to phenol. It was postulated that an enzyme induced by the presence of phenol was necessary to initiate degradation of 4-chlorophenol.

A mathematical model was then developed to describe the dynamic behavior of both substrates in a sequencing batch reactor (SBR). The model utilized rate parameters obtained from batch …


Reactor Design For Hazardous Waste Treatment Using Immobilized Phanerochaete Chrysosporium, Ikram Ul Haq May 1989

Reactor Design For Hazardous Waste Treatment Using Immobilized Phanerochaete Chrysosporium, Ikram Ul Haq

Theses

The degradation of 2-Chlorophenol by Phanerochaete chrysosporium, a white rot fungus, was studied in three different reactor configurations, namely a batch reactor, a chemostatic reactor with the fungus immobilized on a silica based porous biocatalyst, and a packed bed reactor utilizing balsa wood chips as packing.

Preliminary experiments indicated that the use of porous solid support improved the degradation rate in batch reactor and chemostatic reactor. However, the packed bed configuration was found to be superior, and was studied in greater detail. The apparent Michaelis-Menten kinetic constants were obtained for the packed bed reactor.


A Dynamic Model Of A Fill-And-Draw Reactor And Its Implications For Hazardous Waste Treatment, Shing-Hwa Chang May 1988

A Dynamic Model Of A Fill-And-Draw Reactor And Its Implications For Hazardous Waste Treatment, Shing-Hwa Chang

Theses

A dynamic model of a fill-and-draw reactor has been developed, which includes a number of operational parameters such as the draw-down volume, the fraction of total cycle time devoted to fill, reaction, and draw, and the concentration of the toxic substance in the feed to the reactor. The model has been solved numerically for two cases, one assuming Monod kinetics, and one considering substrate inhibition (Andrews kinetics).

The conversions achieved with this type of reactor have been compared to that of a conventional activated sludge (CSTR) design. These results indicate that for most practical settings of the operating parameters, the …