Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Engineering
Use Of A Modified Zirconia Support In The Separation Of Immunoproteins, Anuradha Subramanian, Sabyasachi Sarkar
Use Of A Modified Zirconia Support In The Separation Of Immunoproteins, Anuradha Subramanian, Sabyasachi Sarkar
Papers in Biotechnology
Zirconia beads (25–38 μm in diameter) were modified with N,N,N′,N′-ethylenediaminetetramethylenephosphonic acid to generate a zirconia based pseudoaffinity support, further referred to as r_PEZ. The influence of pH, salt concentration and temperature on the binding of human immunoglobulin G (hIgG) to r_PEZ was studied. Temperature had no significant impact on the maximum binding capacity (Qmax), and the equilibrium-binding constant (Kd), whereas pH and the salt concentration had a noticeable impact on both Qmax and Kd. The Qmax value of 55 mg hIgG/ml of bead was obtained at a pH of 5.5 and found to decrease with an increase of pH. The …
A Comparative Study Of Monoclonal Antibodies (Mabs) Purified From Cell Culture Supernatant On Edtpa-Modified Zirconia Beads And Protein A-Hyper D Support, Anuradha Subramanian, Blanca Martinez, Jill Holm, Peter W. Carr, Clayton V. Mcneff
A Comparative Study Of Monoclonal Antibodies (Mabs) Purified From Cell Culture Supernatant On Edtpa-Modified Zirconia Beads And Protein A-Hyper D Support, Anuradha Subramanian, Blanca Martinez, Jill Holm, Peter W. Carr, Clayton V. Mcneff
Papers in Biotechnology
Colloidal zirconia was spray dried to yield zirconia particles, which were further modified with N, N, N0, N0- Ethylenediamine tetra methylenephosphonic acid (EDTPA) to yield a sup-port for use in bioseparations. EDTPA modified zirconia particles will be further referred to as, r_PEZ. Cell culture supernatants rich in monoclonal antibody (Mab) subtypes IgG1, IgG2a, IgG2b, and IgG3 were chromatographed on a r_PEZ column, and on a protein A-hyper D col-umn that was purchased commercially. All Mab subtypes bound to r_PEZ and process yields in the range of 88 to 99% were obtained. The purity of the Mab products were ascertained by …