Engineering Commons^™

Removal Of Carboxylic Acids And Water From Pyrolysis Oil, George Alexander Marshall

Graduate Theses and Dissertations

Over 70% of the world’s energy consumption is provided by fossil fuels and with those reserves depleting at a fast rate, alternative energy sources or methods are needed to support the world’s energy needs. This research was done in an attempt to make it more economically feasible to produce fuel products, such as bio-diesel, from the upgrading of bio-oil obtained from the pyrolysis of biomass waste material such as sawdust. The high water and oxygenated compound content of bio-oil make it undesirable for fuel use; however, two methods involving surface modified commercial membranes were utilized in hopes of overcoming these …

Membrane Chromatography For Bioseparations: Ligand Design And Optimization, Zizhao Liu

Graduate Theses and Dissertations

Membrane chromatography, or membrane adsorber, represents an attractive alternative to conventional packed bed chromatography used in downstream processing. Membrane chromatography has many advantages, including high productivity, low buffer consumption and ease to scale up. This doctoral dissertation focuses on developing novel polymeric ligands for protein separations using membrane chromatography. Atom transfer radical polymerization (ATRP), known as a controlled radical polymerization technique, has been used to control the architecture of grafted polymeric ligands. The center theme of this dissertation is to develop new polymeric ligands and investigate how the polymer’s property (e.g. flexibility, hydrophobicity) and architecture (e.g. chain density, chain length) …

Initial Structural Subsidence As Reflected In Morrowan And Atokan (Pennsylvanian) Subsurface Stratigraphy, Northern Arkoma Basin, North-Central Arkansas, Fatimah Taher Nahi Al Asadi

Graduate Theses and Dissertations

The Arkoma Basin is a carboniferous foreland basin that is known for its prolific gas production. The objective of this research is to study the initial subsidence of the basin by analyzing the strati-graphic and structural features of the study area. This was accomplished through IHS Petra, Surf-er 13, and Win-Tensor software. Field work studies comprised a large part of this study. Also, a seismic profile and Google earth elevation profiles are among the methods used to study the initial subsidence of the Arkoma Basin. The study area within the basin represents a transitional zone from the shelf into the …

Electrodeionization Versus Electrodialysis: A Clean-Up Of Produced Water In Hydraulic Fracturing, Brigitte Marie Rodgers

Graduate Theses and Dissertations

Electrodeionization (EDI) is a widely studied process ranging from applications in wastewater clean-up in the food and beverage industry to purifying organic compounds. To date, there are no apparent studies on applying this technology to produced wastewater recovered from hydraulic fracking sites. Water consumption within hydraulic fracturing sites can reach in the upwards of millions of gallons per site, so a need for a water recycling process becomes necessary within areas where water requirements are scarce. Implementation of an EDI module that is capable of handling high salt solutions from produced wastewater in subsequent fracturing practices will decrease overall water …

Nanofiltration Rejection Of Contaminants Of Emerging Concern From Municipal Water Resource Recovery Facility Secondary Effluents For Potable Reuse Applications, Steven Michael Jones

Graduate Theses and Dissertations

As reuse of municipal water resource recovery facility (WRRF) effluent becomes vital to augment diminishing fresh drinking water resources, concern exists that conventional barriers may prove deficient and the upcycling of contaminants of emerging concern (CECs) could prove harmful to human health and aquatic species if more effective and robust treatment barriers are not in place.

There are no federal Safe Drinking Water Act (SDWA) regulations in place specifically for direct potable reuse (DPR) of WRRF effluent. Out of necessity, some states are developing their own DPR reuse regulations. Currently, reverse osmosis (RO) is the default full advanced treatment (FAT) …

Cloning, Fed-Batch Expression And Purification Of A Novel Anti-Candida Peptide And Development Of A Cleavage Resistant Variant Of Green Fluorescence Protein, Rudra Palash Mukherjee

Graduate Theses and Dissertations

This work illustrates that the intelligent design of a bioprocess for the production of peptides of various length is possible by having a prior knowledge of desired product, cell line, fermentation conditions, and choices for downstream recovery. Cloning, expression, and recovery of a novel 12-mer anti-Candida peptide served as an illustrative case for a proposed Escherichia coli platform. The antifungal peptide, expressed as a fusion to GFPUV during high call density fed-batch, was recovered from cell lysate, concentrated using ion exchange chromatography, and finally cleaved with cyanogen bromide. The platform was capable of producing active peptide capable of arresting the …