Engineering Commons^™

Turbulent Mixing And Chemical Reaction In Baffled Stirred Tank Reactors : A Comparison Between Experiments And A Novel Micromixing-Based Computational Fluid Dynamics Model, Otute Akiti

Dissertations

The optimization of reaction processes to maximize the yield of a desired product while minimizing the formation of undesired by-products is one of the most important steps in process development for drug manufacturing or fine chemical production. In many situations the kinetics of product formation can be quite complex and involve a number of intermediate steps as well as parallel and serial reactions. This renders the systems sensitive to the operating conditions. Often, upon scale-up, a decrease in the yield of the desired product is experienced, while more undesired by-products are produced. A large number of interrelated variables influence the …

The Impact And Fate Of Aqueous Sodium Nitrate On Hydrocarbon Flames, Ann Marie Flynn

Dissertations

This study examined the impact and fate of sodium (fed as an aqueous nitrate solution) in fuel-rich methane/air and methane/methyl chloride/air flames as a function of equivalence ratios that experienced diffusion of air from the surroundings. The flames were stabilized on a slotted, uncooled burner. The data set was divided into profiles of relative sodium atom concentrations, temperatures, and selected stable species concentrations. The flames were simulated using a modified version of the Sandia FORTRAN program for modeling steady laminar one-dimensional premixed flames complete with detailed mechanisms. The results showed that maximum sodium atom concentration in the flame is decreased …

Endotoxin Removal From Protein Solutions By Immobilized Metal Ion Affinity Chromatography, Yun Kang

Dissertations

In the biotechnology/pharmaceutical industry, the removal of endotoxins from final protein product has always been a challenge. A novel method for endotoxin removal from protein solutions, immobilized metal (ion) affinity chromatography (IMAC) has been developed in this study. Fe³⁺ was charged to a chelating gel that contains iminodiacetic acid (IDA). The IDA- Fe³⁺ IMAC column was exploited to remove endotoxin from protein solutions. This method involves the adsorption of the endotoxin on IMAC column. More than 99% endotoxin is removed and protein yield is higher than 90%. IDA-Fe³⁺ IMAC has proved to be an economical and efficient …

Fluidization And Soot Filtration In A Rotating Fluidized Bed, Gui-Hua Qian

Dissertations

A horizontal rotating fluidized bed (RFB) has been investigated for the removal of soot from diesel engine exhaust. This requires necessitating an understanding of the nature of fluidization, particle mixing and filtration in the RFB.

In an RFB, particles are subject to a centrifugal force which can be much larger than the force of gravity. Therefore the Geldart classification for conventional fluidized beds must be modified for rotating fluidized beds. A theoretical analysis shows that group A particles (minimum bubbling velocity U_mb > minimum fluidization velocity U_mf can shift to group B U_mb = U_mf and group …

An Integrated Membrane-Based Chromatographic Process For Biomolecule Isolation And Purification, Xiaoping Dai

Dissertations

Biomolecules obtained from fermentation and cell-culture processes are present in low concentrations in a complex medium/broth. For intracellular products, cells are first harvested. Subsequently, cell lysis and homogenization are undertaken to produce a homogenate. The biomolecules are next separated from the cell debris by lysate clarification. For extracellular products, the biomolecules are separated from the whole cells by clarification. The overall yield is reduced with each additional step. As the cost of bioseparation process accounts for 50-80% of the final product cost, innovations in and improvement of bio-downstream processing are greatly needed.

In this research, a cyclic process integrating a …

In-Line Measurement Of Solibility Of Physical Blowing Agents In Thermoplastic Melts As Related To Extrusion Foaming, Qiang Zhang

Dissertations

A novel in-line method for measuring physical blowing agent solubility in thermoplastic melts in extrusion foaming equipment has been developed in this study. This method uses video microscopy in connection with a foaming extruder (single or twin-screw) to generate solubility data by observing the onset of gas bubble formation/dissolution. This method offers a number of advantages over the existing conventional methods. It can be easily used to obtain solubility data for many foamable thermoplastic-physical blowing agent systems at high pressure and temperature near actual foaming conditions. Data generated using this method could be directly used to characterize and control a …