Engineering Commons^™

Tissue Engineering Scaffolds With Enhanced Oxygen Delivery Using A Cyclodextrin Inclusion Complex, Tierney Gb Deluzio

Electronic Thesis and Dissertation Repository

The development of a strategy to improve oxygen delivery to cells seeded on scaffolds is essential for the success of tissue engineering applications. The focus of this work was to explore the application of cyclodextrin inclusion complexes (CD:ICs) with perfluorocarbons as oxygen carriers. CD:ICs were prepared from alpha-cyclodextrin and perfluoroperhydrophenanthrene via co-precipitation, paste mixing, and dry mixing complexation techniques. Characterization indicated that paste mixing at a 2:1 host:guest ratio was the most effective method for complexation between the parent molecules. The CD:ICs were then successfully incorporated in 3D fibrous mats via electrospinning with poly(carbonate urethane) and polycaprolactone as biostable and …

Electrospinning Of Core-Shell Collagen Nanofibers, Ying Li

Electronic Thesis and Dissertation Repository

In tissue engineering, the scaffold plays a critical role in guiding and supporting cells to function and grow optimally. The electrospun nanofibrous scaffold can serve as a near ideal substrate for tissue engineering because it has high surface area and the three-dimensional interconnected porous network can enhance cell attachment and proliferation. Core-shell nanofibrous scaffolds produced with coaxial electrospinning allow bioactive molecule encapsulation to improve cell adhesion, mediate and promote the proper signaling among the cells for their functioning and growth. In the current study, core-shell collagen nanofibers were fabricated via coaxial electrospinning with horizontal and vertical configurations. Core-shell nanofibers with …

Sol-Gel Derived Biodegradable And Bioactive Organic-Inorganic Hybrid Biomaterials For Bone Tissue Engineering, Bedilu A. Allo

Electronic Thesis and Dissertation Repository

Treatments of bone injuries and defects have been largely centered on replacing the lost bone with tissues of allogeneic or xenogeneic sources as well as synthetic bone substitutes, which in all lead to limited degree of structural and functional recovery. As a result, tissue engineering has emerged as a viable technology to regenerate the structures and therefore recover the functions of bone tissue rather than replacement alone. Hence, the current strategies of bone tissue engineering and regeneration rely on bioactive scaffolds to mimic the natural extracellular matrix (ECM) as templates onto which cells attach, multiply, migrate and function.

In this …

Oxygen Delivery Strategies In Tissue-Engineering Constructs, Dawit Gezahegn Seifu

Electronic Thesis and Dissertation Repository

The supply of nutrients and the removal of waste products play a major role in tissue engineering. From all the nutrients necessary for cells seeded on scaffolds for tissue regeneration, oxygen is the limiting component due to its low solubility in culture media while cells consume five to six moles of oxygen for every mole of monosaccharide. The aim of the present work was to develop different strategies to improve the supply of oxygen to human coronary artery smooth muscle cells (HCASMC) seeded on three dimensional (3D) porous biostable polyurethane scaffolds. As a springboard for the study, the measured value …