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Development Of Steady-State And Dynamic Flux Models For Broad-Scope Microbial Metabolism Analysis, Lian He
Development Of Steady-State And Dynamic Flux Models For Broad-Scope Microbial Metabolism Analysis, Lian He
McKelvey School of Engineering Theses & Dissertations
Flux analysis techniques, including flux balance analysis (FBA) and 13C-metabolic flux analysis (MFA), can characterize carbon and energy flows through a cells metabolic network. By employing both 13C-labeling experiments and nonlinear programming, 13C-MFA provides a rigorous way of examining cell flux distributions in the central metabolism. FBA, on the other hand, gives a holistic review of optimal fluxomes on the genome scale. In this dissertation, flux analysis techniques were constructed to investigate the microbial metabolisms.
First, an open-source and programming-free platform of 13C-MFA (WUFlux) with a user-friendly interface in MATLAB was developed, which allowed both mass isotopomer distribution (MID) analysis …