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Full-Text Articles in Engineering

Using Elastin-Like Polypeptides For Better Retention Of Biofuels, Yu Hong Wang, Ethan T. Hilman, Kevin V. Solomon Aug 2016

Using Elastin-Like Polypeptides For Better Retention Of Biofuels, Yu Hong Wang, Ethan T. Hilman, Kevin V. Solomon

The Summer Undergraduate Research Fellowship (SURF) Symposium

Elastin-like polypeptides (ELPs) are synthetic molecules that exhibit an interesting property of inverse temperature phase transition; they exist as soluble monomers at low temperatures and form insoluble aggregates at higher temperatures. The transition temperature depends on the pH, salt concentration, and the amino acid sequence of the ELP. This unique and reversible behavior, along with their high biocompatibility has made them a strategic tool for various biomedical applications. However, their hydrophobic properties also make them a prime candidate for biofuel production. As high levels of many commercially important organic solvents are toxic to the cells that make them, ELPs can …


Minimizing Ethanol Concentration In Organosolv Pretreatment For The Saccharification Of Loblolly Pine, Nelson B. Heringer May 2016

Minimizing Ethanol Concentration In Organosolv Pretreatment For The Saccharification Of Loblolly Pine, Nelson B. Heringer

Biological and Agricultural Engineering Undergraduate Honors Theses

Organic solvent pretreatment, commonly known as organosolv, is a method used to prepare biomass for enzymatic hydrolysis for the production of biofuels. This method common uses ethanol as the organic solvent. However, this creates an economic issue with the product stream, as ethanol becomes a product and input. This project sought to explore how decreasing the use of ethanol in organosolv pretreatment affected the recoverability of sugars after enzymatic hydrolysis. Loblolly pine (Pinus taeda L.) was pretreated at 170 °C for 60 minutes in 1% dilute sulfuric acid and an ethanol concentration varying from 65% to 35%. Compositional …


Induction Of Cellulase In High Solids Cultivation Of Trichoderma Reesei For Enhanced Enzymatic Hydrolysis Of Lignocellulose, Danielle Empson Jan 2016

Induction Of Cellulase In High Solids Cultivation Of Trichoderma Reesei For Enhanced Enzymatic Hydrolysis Of Lignocellulose, Danielle Empson

Theses and Dissertations--Biosystems and Agricultural Engineering

This project aimed investigated cellulase in-situ production for large-scale on-farm production of lignocellulosic biofuel. Cellulase activity and glucose released by T. reesei with corn stover and wheat bran as co-substrates for solid state cultivation (SSC) were examined. Co-cultivation has previously increased T. reesei cellulase, but corn stover and wheat bran have not been co-cultivated (Dhillon, Oberoi et al. 2011). This work compared cellulase activity and glucose concentration of corn stover co-cultivated with 0-40% wheat bran in high solids. Samples with at least 20% wheat bran exhibited increased cellulase activity. However, the average glucose concentration without wheat bran was 3.29 g/L …