Engineering Commons^™

Mapping The Membrane Proteome Of Anaerobic Gut Fungi Identifies A Wealth Of Carbohydrate Binding Proteins And Transporters, Susanna Seppälä, Kevin V. Solomon, Sean P. Gilmore, John K. Henske, Michelle A. O'Malley

School of Agricultural & Biological Engineering Faculty Publications

Background

Engineered cell factories that convert biomass into value-added compounds are emerging as a timely alternative to petroleum-based industries. Although often overlooked, integral membrane proteins such as solute transporters are pivotal for engineering efficient microbial chassis. Anaerobic gut fungi, adapted to degrade raw plant biomass in the intestines of herbivores, are a potential source of valuable transporters for biotechnology, yet very little is known about the membrane constituents of these non-conventional organisms. Here, we mined the transcriptome of three recently isolated strains of anaerobic fungi to identify membrane proteins responsible for sensing and transporting biomass hydrolysates within a competitive and …