Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Publication
- Publication Type
Articles 1 - 8 of 8
Full-Text Articles in Engineering
An Assessment Of Blood Vessel Remodeling Of Nanofibrous Poly(Ε-Caprolactone) Vascular Grafts In A Rat Animal Model, Jana Horakova, Tereza Blassova, Zbynek Tonar, Connor Mccarthy, Katerina Strnadova, David Lukas, Petr Mikes, Patrick Bowen, Roger J. Guillory Ii, Megan C. Frost, Jeremy Goldman
An Assessment Of Blood Vessel Remodeling Of Nanofibrous Poly(Ε-Caprolactone) Vascular Grafts In A Rat Animal Model, Jana Horakova, Tereza Blassova, Zbynek Tonar, Connor Mccarthy, Katerina Strnadova, David Lukas, Petr Mikes, Patrick Bowen, Roger J. Guillory Ii, Megan C. Frost, Jeremy Goldman
Michigan Tech Publications
The development of an ideal vascular prosthesis represents an important challenge in terms of the treatment of cardiovascular diseases with respect to which new materials are being considered that have produced promising results following testing in animal models. This study focuses on nanofibrous polycaprolactone-based grafts assessed by means of histological techniques 10 days and 6 months following suturing as a replacement for the rat aorta. A novel stereological approach for the assessment of cellular distribution within the graft thickness was developed. The cellularization of the thickness of the graft was found to be homogeneous after 10 days and to have …
3d Bioprinting Hydrogel For Tissue Engineering An Ascending Aortic Scaffold, Benjamin Stewart
3d Bioprinting Hydrogel For Tissue Engineering An Ascending Aortic Scaffold, Benjamin Stewart
Electronic Theses and Dissertations
The gold standard in 2016 for thoracic aortic grafts is Dacron®, polyethylene terephthalate, due to the durability over time, the low immune response elicited and the propensity for endothelialization of the graft lumen over time. These synthetic grafts provide reliable materials that show remarkable long term patency. Despite the acceptable performance of Dacron® grafts, it is noted that autographs still outperform other types of vascular grafts when available due to recognition of the host's cells and adaptive mechanical properties of a living graft. 3-D bioprinting patient-specific scaffolds for tissue engineering (TE) brings the benefits of non-degrading synthetic …
Biological Scaffolds For Peripheral Vascular Surgery, George Fercana
Biological Scaffolds For Peripheral Vascular Surgery, George Fercana
All Dissertations
The gold standards for small diameter peripheral vascular graft replacement are autologous arteries or veins; however, one-third of patients lack such vessels due to previous vessel harvesting or advanced vascular disease. A promising approach for patients in this category is tissue engineering with off-the-shelf biological vascular grafts. Three small diameter acellular scaffolds were developed and evaluated as vascular grafts. Porcine renal arteries (2-3 mm diameter, 20 mm length) were decellularized by immersion and stabilized with penta-galloyl glucose (PGG) with and without subsequent heparinization via carbodiimide chemistry. Bovine mammary (4-6 mm ID, 250 mm length) and femoral arteries (6-8 mm ID, …
Evaluation Of Decellularization Procedures For Porcine Arteries, Charles Clapp
Evaluation Of Decellularization Procedures For Porcine Arteries, Charles Clapp
Biomedical Engineering
Coronary artery disease has become the leading cause of death in the United States, with over 425,000 deaths in 2006. Stenting has evolved into the preferred preventative technique for myocardial infarction by opening up an occluded artery, due to its low invasiveness compared to the alternative of coronary artery bypass grafting. Bare metal stents have been improved by coating with anti-proliferative drugs to advance their effects, but even drug eluting stents still have a risk of restenosis, thrombus formation, and necessary revascularization. Continual advancement in stent design necessitates faster, effective pre-clinical evaluation techniques. Kristen Cardinal, Ph.D., developed the blood vessel …
Optimization Of A Tri-Layered Vascular Graft: The Influence Of Cellular And Mechanical Properties, Michael Mcclure
Optimization Of A Tri-Layered Vascular Graft: The Influence Of Cellular And Mechanical Properties, Michael Mcclure
Theses and Dissertations
Electrospinning is a polymer processing technique which allows for the production of nano to micro size fibers and scaffolds which can be composed of numerous synthetic biodegradable materials and natural biopolymers. Natively, elastin and collagen are the main components of vascular tissue. Arranged in a tri-layered structure, they create a specific mechanical environment that can withstand the rigors of circulation. The goal of this study was to develop a mechanically ‘biomimicking’ vascular graft composed of three distinct layers through the process of electrospinning. We hypothesize that the use of bioactive agents such as elastin, collagen, and silk to supplement poly(caprolactone) …
Novel Nanofiber Structures And Advanced Tissue Engineering Applications, Vince Beachley
Novel Nanofiber Structures And Advanced Tissue Engineering Applications, Vince Beachley
All Dissertations
Extracellular matrix (ECM) nanofibers such as collagen and elastin make up an important component of natural tissues. These structural components serve to impart mechanical strength and provide locations for cell attachment and biomolecule storage. Cells respond to their structural environment in a wide variety of ways beyond physical support, and it has been demonstrated that this environment directly modulates cell behaviors such as, morphology, differentiation, ECM production, attachment, and migration. ECM nanofibers also play an important role as a template for tissue formation during development, remodeling, and regeneration. Nanofiber based tissue engineering strategies aim to mimic the geometry of the …
Quantitative Scanning Electron Microscopy (Sem) To Study The Adhesion And Spreading Of Human Endothelial Cells To Surface-Modified Poly(Carbonate Urethane)S, C. James Kirkpatrick, Tillmann Otterbach, Dirk Anderheiden, Johannes Schiefer, Horst Richter, Hartwig Hartwig Hocker, Christian Mittermayer, Albert Dekker
Quantitative Scanning Electron Microscopy (Sem) To Study The Adhesion And Spreading Of Human Endothelial Cells To Surface-Modified Poly(Carbonate Urethane)S, C. James Kirkpatrick, Tillmann Otterbach, Dirk Anderheiden, Johannes Schiefer, Horst Richter, Hartwig Hartwig Hocker, Christian Mittermayer, Albert Dekker
Cells and Materials
The polymers currently in use as vascular prostheses are, in the native state, poor substrates for endothelial cell (EC) adhesion and growth. This has a negative effect on the success of pre-seeding regimes. One attempt to improve the polymer substrate is to covalently couple reactive molecules to the surface, which can be used as anchorage points for EC or serve as spacer molecule to couple biological signal molecules such as oligopeptides. We have used a digitized image analysis system coupled to a scanning electron microscope to study the adhesion and spreading of human venous EC to unmodified poly(carbonate urethane) (uPCU), …
Patency Of Small Caliber, Superhydrophobic E-Ptfe Vascular Grafts; A Pilot Study In The Rabbit Carotid Artery, J. M. Schakenraad, I. Stokroos, H. Bartels, H. J. Busscher
Patency Of Small Caliber, Superhydrophobic E-Ptfe Vascular Grafts; A Pilot Study In The Rabbit Carotid Artery, J. M. Schakenraad, I. Stokroos, H. Bartels, H. J. Busscher
Cells and Materials
e-PTFE vascular grafts (Gore, internal diameter 1.5 mm, pore size 30 μm) were luminally treated by ionbeam etching followed by oxygen glow-discharge, to obtain a superhydrophobic luminal surface. Water contact angles of thus modified sheet material were 140-150°, which is significantly higher than on non-treated sheet PTFE (109°). These modified vascular grafts were implanted in the rabbit carotid artery, using an end to end anastomotic technique. After two hours, the grafts were still patent and the wounds were closed. One week after the implantation, the prostheses were still patent. Subsequently, the grafts were harvested and prepared for light microscopy, transmission …