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In Vitro, Non-Invasive Imaging And Detection Of Single Living Mammalian Cells Interacting With Bio-Nano-Interfaces, Qifei Li

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Study of bio-nano-interfaces of living mammalian cells will help the identification of cellular alterations (e.g. nucleic acids, amino acids, biomechanics, etc.) due to external stimuli, the design of biomaterials (e.g. nanoparticles, nanotubes) and the investigation on the interaction between cells and bio-nano-interfaces (e.g. cell differentiation on 3D nanostructured materials). The chemical, physical, and mechanical changes of mammalian cells interacting with biomaterials can be investigated by analytical techniques. In this dissertation, cellular responses and property changes are evaluated by non-invasive spectroscopic technique, Raman microscopy (RM), and atomic force microscopy (AFM) combined with traditional biological methods. This dissertation includes the biophysical, biochemical …