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Full-Text Articles in Engineering
Characterization Of Tissue-Specific Matrix-Derived Bioscaffolds For Nucleus Pulposus Cell Culture, Marco A. Herrera Quijano
Characterization Of Tissue-Specific Matrix-Derived Bioscaffolds For Nucleus Pulposus Cell Culture, Marco A. Herrera Quijano
Electronic Thesis and Dissertation Repository
Bioscaffolds derived from the extracellular matrix (ECM) have shown the capacity to promote regeneration by providing tissue-specific biological instructive cues that can enhance cell survival and direct lineage-specific differentiation. This study focused on the development and characterization of two-dimensional (2-D) and three-dimensional (3-D) cell culture platforms incorporating decellularized nucleus pulposus (DNP). First, a novel detergent-free protocol was developed for decellularizing bovine NP tissues that was effective at removing cellular content while preserving key ECM constituents including collagens and glycosaminoglycans. Culture studies showed that 2-D coatings derived from the DNP could support cell attachment but did not maintain or rescue the …
Synthesis And Performance Testing Of Ecm Fiber Scaffolds, Cassandra Reed
Synthesis And Performance Testing Of Ecm Fiber Scaffolds, Cassandra Reed
Graduate Theses and Dissertations
The progression of regenerative medicine has advanced the treatment of multiple illnesses and injuries throughout the years. A good example of the benefits of this research is the work that has gone into volumetric muscle loss (VML), where more than 20% of the muscle is loss. Skeletal muscle makes up 40% of the human body so a loss of that size greatly diminishes the strength, the flexibility, physiology, and quality of life of the injured individual. For that reason, various techniques are used to counteract the loss of structure and innate cellular signaling in order to circumvent that from happening. …
Evaluating The Effects Of Wood Source On The Physicochemical Properties Of Crosslinked Cellulose Nanocrystals, Helena Tchoungang Nkeumen
Evaluating The Effects Of Wood Source On The Physicochemical Properties Of Crosslinked Cellulose Nanocrystals, Helena Tchoungang Nkeumen
Graduate Theses and Dissertations
Cellulose is an abundant and naturally occurring biopolymer that has been used by humans for food, shelter, and clothing for about two centuries now. Highly crystalline nanoparticles derived from cellulose, called cellulose nanocrystals (CNCs), show great potential to meet the rising need for sustainable and nontoxic materials for biomedical applications. However, multiple biomedical applications of CNCs, such as those involving their use in tissue engineering scaffolds, require CNC-based structures to be stable in aqueous environments, a property that native CNCs do not possess due to their inherent hydrophilicity. Chemical crosslinking of CNCs addresses this issue by providing aqueous stability to …
Applied Machine Learning In Extrusion-Based Bioprinting, Shuyu Tian
Applied Machine Learning In Extrusion-Based Bioprinting, Shuyu Tian
Theses and Dissertations
Optimization of extrusion-based bioprinting (EBB) parameters have been systematically conducted through experimentation. However, the process is time and resource-intensive and not easily translatable across different laboratories. A machine learning (ML) approach to EBB parameter optimization can accelerate this process for laboratories across the field through training using data collected from published literature. In this work, regression-based and classification-based ML models were investigated for their abilities to predict printing outcomes of cell viability and filament diameter for cell-containing alginate and gelatin composite hydrogels. Regression-based models were investigated for their ability to predict suitable extrusion pressure given desired cell viability when keeping …
Carbon Nanotube-Coated Scaffolds For Tissue Engineering Applications, Soham Dipakbhai Parikh
Carbon Nanotube-Coated Scaffolds For Tissue Engineering Applications, Soham Dipakbhai Parikh
Browse all Theses and Dissertations
Carbon Nanotubes (CNTs) have beneficial properties for cell scaffolding, which has translated into effective growth of bone, muscle, and cardiac cells. However, loose carbon nanotubes can cause in vivo toxicity. To reduce this risk, our team has developed biomimetic scaffolds with multiscale hierarchy where carpet-like CNT arrays are covalently bonded to larger biocompatible substrates. In this study, we have tested such scaffolds in two distinct types of biomedical applications involving glioblastoma and keratinocyte cells. The growth of glioblastoma (GBM) cells on our CNT-coated biomimetic scaffolds was evaluated to check their suitability as a potential chemotherapy-loaded implant for GBM patient treatment. …