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Articles 1 - 8 of 8
Full-Text Articles in Engineering
A Case Study: Data Management In Biomedical Engineering, Glenn Gaudette, Donna Kafel
A Case Study: Data Management In Biomedical Engineering, Glenn Gaudette, Donna Kafel
Glenn R. Gaudette
In a biomedical engineering lab at Worcester Polytechnic Institute, co-author Dr. Glenn R. Gaudette and his research team are investigating the effects of stem cell therapy on the regeneration of function in damaged cardiac tissue in laboratory rats. Each instance of stem cell experimentation on a rat yields hundreds of data sets that must be carefully captured, documented and securely stored so that the data will be easily accessed and retrieved for papers, reports, further research, and validation of findings, while meeting NIH guidelines for data sharing. After a brief introduction to the bioengineering field and stem cell research, this …
Science Panel Discussion Presentation: "Data Management In Biomedical Engineering: Needs And Implementation", Glenn Gaudette
Science Panel Discussion Presentation: "Data Management In Biomedical Engineering: Needs And Implementation", Glenn Gaudette
Glenn R. Gaudette
Glenn Gaudette, PhD, is an Associate Professor, Department of Biomedical Engineering, Worcester Polytechnic Institute. He discussed data management in his research laboratory, which is focused on regenerating mechanical and electrophysiological function in hearts with cardiovascular disease.
Development And Characterization Of A 3d Multicell Microtissue Culture Model Of Airway Smooth Muscle., Glenn Gaudette, Adrian West, Nishat Zaman, Darren Cole, Matthew Walker, Wesley Legant, Thomas Boudou, Christopher Chen, John Favreau, Elizabeth Cowley, Geoffrey Maksym
Development And Characterization Of A 3d Multicell Microtissue Culture Model Of Airway Smooth Muscle., Glenn Gaudette, Adrian West, Nishat Zaman, Darren Cole, Matthew Walker, Wesley Legant, Thomas Boudou, Christopher Chen, John Favreau, Elizabeth Cowley, Geoffrey Maksym
Glenn R. Gaudette
Airway smooth muscle (ASM) cellular and molecular biology is typically studied with single-cell cultures grown on flat 2D substrates. However, cells in vivo exist as part of complex 3D structures, and it is well established in other cell types that altering substrate geometry exerts potent effects on phenotype and function. These factors may be especially relevant to asthma, a disease characterized by structural remodeling of the airway wall, and highlights a need for more physiologically relevant models of ASM function. We utilized a tissue engineering platform known as microfabricated tissue gauges to develop a 3D culture model of ASM featuring …
Enhanced Recovery Of Mechanical Function In The Canine Heart By Seeding An Extracellular Matrix Patch With Mesenchymal Stem Cells Committed To A Cardiac Lineage., Glenn Gaudette, Irina Potapova, Sergey Doronin, Damon Kelly, Amy Rosen, Adam Schuldt, Zhongju Lu, Paul Kochupura, Richard Robinson, Michael Rosen, Peter Brink, Ira Cohen
Enhanced Recovery Of Mechanical Function In The Canine Heart By Seeding An Extracellular Matrix Patch With Mesenchymal Stem Cells Committed To A Cardiac Lineage., Glenn Gaudette, Irina Potapova, Sergey Doronin, Damon Kelly, Amy Rosen, Adam Schuldt, Zhongju Lu, Paul Kochupura, Richard Robinson, Michael Rosen, Peter Brink, Ira Cohen
Glenn R. Gaudette
The need to regenerate tissue is paramount, especially for the heart that lacks the ability to regenerate after injury. The urinary bladder extracellular matrix (ECM), when used to repair a right ventricular defect, successfully regenerated some mechanical function. The objective of the current study was to determine whether the regenerative effect of ECM could be improved by seeding the patch with human mesenchymal stem cells (hMSCs) enhanced to differentiate down a cardiac linage. hMSCs were used to form three-dimensional spheroids. The expression of cardiac proteins was determined in cells exposed to the spheroid formation and compared with nonmanipulated hMSCs. To …
The Use Of Extracellular Matrix As An Inductive Scaffold For The Partial Replacement Of Functional Myocardium., Glenn Gaudette, S Badylak, P Kochupura, I Cohen, S Doronin, A Saltman, T Gilbert, D Kelly, R Ignotz
The Use Of Extracellular Matrix As An Inductive Scaffold For The Partial Replacement Of Functional Myocardium., Glenn Gaudette, S Badylak, P Kochupura, I Cohen, S Doronin, A Saltman, T Gilbert, D Kelly, R Ignotz
Glenn R. Gaudette
Regenerative medicine approaches for the treatment of damaged or missing myocardial tissue include cell-based therapies, scaffold-based therapies, and/or the use of specific growth factors and cytokines. The present study evaluated the ability of extracellular matrix (ECM) derived from porcine urinary bladder to serve as an inductive scaffold for myocardial repair. ECM scaffolds have been shown to support constructive remodeling of other tissue types including the lower urinary tract, the dermis, the esophagus, and dura mater by mechanisms that include the recruitment of bone marrow-derived progenitor cells, angiogenesis, and the generation of bioactive molecules that result from degradation of the ECM. …
Myocardial Recovery From Ischemia Is Impaired In Cd36-Null Mice And Restored By Myocyte Cd36 Expression Or Medium-Chain Fatty Acids., Glenn Gaudette, H Irie, I Krukenkamp, J Brinkmann, A Saltman, W Jou, J Glatz, N Abumrad, A Ibrahimi
Myocardial Recovery From Ischemia Is Impaired In Cd36-Null Mice And Restored By Myocyte Cd36 Expression Or Medium-Chain Fatty Acids., Glenn Gaudette, H Irie, I Krukenkamp, J Brinkmann, A Saltman, W Jou, J Glatz, N Abumrad, A Ibrahimi
Glenn R. Gaudette
Long-chain fatty acid uptake, which provides a large part of myocardial energy, is impaired in human and murine hearts deficient in the membrane fatty acid translocase, FAT/CD36. We examined myocardial function in CD36-null mice using the working heart. Fatty acid oxidation and stores of glycogen, triglycerides, and ATP were reduced in CD36-deficient hearts and were restored to WT levels by rescue of myocyte CD36. Under normal perfusion conditions, CD36-null hearts had similar cardiac outputs and end-diastolic pressures as WT or transgenic hearts. After 6 min of ischemia, cardiac output decreased by 41% and end diastolic pressure tripled for CD36-null hearts, …
Isoform-Specific Stimulation Of Cardiac Na/K Pumps By Nanomolar Concentrations Of Glycosides., Glenn Gaudette, Junyuan Gao, Randy Wymore, Yongli Wang, Irvin Krukenkamp, Ira Cohen, Richard Mathias
Isoform-Specific Stimulation Of Cardiac Na/K Pumps By Nanomolar Concentrations Of Glycosides., Glenn Gaudette, Junyuan Gao, Randy Wymore, Yongli Wang, Irvin Krukenkamp, Ira Cohen, Richard Mathias
Glenn R. Gaudette
It is well-known that micromolar to millimolar concentrations of cardiac glycosides inhibit Na/K pump activity, however, some early reports suggested nanomolar concentrations of these glycosides stimulate activity. These early reports were based on indirect measurements in multicellular preparations, hence, there was some uncertainty whether ion accumulation/depletion rather than pump stimulation caused the observations. Here, we utilize the whole-cell patch-clamp technique on isolated cardiac myocytes to directly measure Na/K pump current (I(P)) in conditions that minimize the possibility of ion accumulation/depletion causing the observed effects. In guinea pig ventricular myocytes, nanomolar concentrations of dihydro-ouabain (DHO) caused an outward current that appeared …
The Treatment Of Depression With Group Behavioural-Cognitive Therapy And Imipramine., Glenn Gaudette, A Stravynski, R Verreault, R Langlois, S Gagnier, M Larose
The Treatment Of Depression With Group Behavioural-Cognitive Therapy And Imipramine., Glenn Gaudette, A Stravynski, R Verreault, R Langlois, S Gagnier, M Larose
Glenn R. Gaudette
Twenty-four outpatients meeting DSM-III-R criteria for major depression were assigned to group behavioral-cognitive therapy either with or without antidepressant medication (imipramine). Eighteen patients completed 15 weekly sessions of treatment. Equivalent improvement was observed in both regimens after treatment. The results essentially maintained at six months follow-up. This suggests that a group format of behavioural-cognitive therapy is a viable therapeutic intervention for outpatients diagnosed as suffering from major depression. However, the addition of imipramine to group behavioural-cognitive therapy did not enhance the outcome.