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Characterization Of Tissue-Specific Matrix-Derived Bioscaffolds For Nucleus Pulposus Cell Culture, Marco A. Herrera Quijano
Characterization Of Tissue-Specific Matrix-Derived Bioscaffolds For Nucleus Pulposus Cell Culture, Marco A. Herrera Quijano
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Bioscaffolds derived from the extracellular matrix (ECM) have shown the capacity to promote regeneration by providing tissue-specific biological instructive cues that can enhance cell survival and direct lineage-specific differentiation. This study focused on the development and characterization of two-dimensional (2-D) and three-dimensional (3-D) cell culture platforms incorporating decellularized nucleus pulposus (DNP). First, a novel detergent-free protocol was developed for decellularizing bovine NP tissues that was effective at removing cellular content while preserving key ECM constituents including collagens and glycosaminoglycans. Culture studies showed that 2-D coatings derived from the DNP could support cell attachment but did not maintain or rescue the …