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Effects Of Micro-Features On Cell Detachment From Poly(N-Isopropylacrylamide) Coated Polydimethylsiloxane Membranes, Luke Webel
Williams Honors College, Honors Research Projects
The tested hypothesis was that features on polydimethylsiloxane (PDMS) surfaces coated with a poly(N-isopropylacrylamide)/aminopropyltriethoxysilane or pNIPAAM/APTES thin film would accelerate cell detachment than the film coated on a unfeatured surface. This project tested samples with features generated by molds, wrinkling, and sandpaper roughened substrates. Surface feature generation methods were limited to mechanical means, and characterized by microscopy and strain rates. 50/50 mixtures of 1.5 wt.% pNIPAAM/ APTES were used to coat thin films (30-40 nm) on PDMS membranes by spin-coating, and the coated membranes were thermally annealed to chemically graft pNIPAAm/APTES on the membrane and their thermo-responsive property was assessed …
Modification Of Bacterial Cellulose Using Organosilanes To Enhance Cell Adhesion And Growth, Kristi Ferrato
Modification Of Bacterial Cellulose Using Organosilanes To Enhance Cell Adhesion And Growth, Kristi Ferrato
Williams Honors College, Honors Research Projects
In this project, drying bacterial cellulose (BC) to maintain its structural integrity, and treating BC with organosilanes 3-aminopropyltriethoxysilane (APTES) and (3-Mercaptopropyl)trimethoxysilane (MPTMS) as well as with various concentration of gelatin to increase the cells per area was studied. Drying BC with hexamethyldisilazane (HMDS) by soaking the BC in ascending concentrations of ethanol and HMDS did not protect the structural integrity of the BC grown in lab but did for the BC grown in Dr. Siriporn Taokaew’s lab. Freeze drying also did not fully protect the structural integrity as the BC was placed in a freezer for several hours at -20oC …