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Full-Text Articles in Engineering
Cell Culture Method And Apparatus For Mechanically Stimulating Cells, Seth W. Donahue
Cell Culture Method And Apparatus For Mechanically Stimulating Cells, Seth W. Donahue
Michigan Tech Patents
A cell culture assembly and a method for culturing cells that provide mechanical stimulation to cells. The cell culture assembly can include a flow chamber positioned in a fluid path and a support comprising cells positioned within the flow chamber to expose the cells to the fluid path. The cell culture assembly can further include a means for producing a steady flow of fluid in the fluid path, and a means for producing an oscillatory flow of fluid in the fluid path simultaneously with producing the steady flow of fluid in the fluid path to mechanically stimulate the cells. The …
Isolated Cellulose Synthase Promoter Regions, Chandrashekhar P. Joshi
Isolated Cellulose Synthase Promoter Regions, Chandrashekhar P. Joshi
Michigan Tech Patents
The invention relates to methods of inducing expression of coding sequences including cellulose synthase coding sequences in transgenic plants using promoters of cellulose synthase genes from Populus plant species and transgenic plants produced by the methods.
Exploration Of The Role Of Serum Factors In Maintaining Bone Mass During Hibernation In Black Bears, Rachel Marie Bradford
Exploration Of The Role Of Serum Factors In Maintaining Bone Mass During Hibernation In Black Bears, Rachel Marie Bradford
Dissertations, Master's Theses and Master's Reports - Open
Disuse osteoporosis is a condition in which reduced mechanical loading (e.g. bed-rest, immobilization, or paralysis) results in unbalanced bone turnover. The American black bear is a unique, naturally occurring model for the prevention of disuse osteoporosis. Bears remain mostly inactive for up to half a year of hibernation annually, yet they do not lose bone mechanical strength or structural properties throughout hibernation. The long-term goal of this study is to determine the biological mechanism through which bears maintain bone during hibernation. This mechanism could pinpoint new signaling pathway targets for the development of drugs for osteoporosis prevention. In this study, …
Use Of A 3d Perfusion Bioreactor With Osteoblasts And Osteoblast/Endothelial Cell Co-Cultures To Improve Tissue-Engineered Bone, Matthew J. Barron
Use Of A 3d Perfusion Bioreactor With Osteoblasts And Osteoblast/Endothelial Cell Co-Cultures To Improve Tissue-Engineered Bone, Matthew J. Barron
Dissertations, Master's Theses and Master's Reports - Open
The delivery of oxygen, nutrients, and the removal of waste are essential for cellular survival. Culture systems for 3D bone tissue engineering have addressed this issue by utilizing perfusion flow bioreactors that stimulate osteogenic activity through the delivery of oxygen and nutrients by low-shear fluid flow. It is also well established that bone responds to mechanical stimulation, but may desensitize under continuous loading. While perfusion flow and mechanical stimulation are used to increase cellular survival in vitro, 3D tissue-engineered constructs face additional limitations upon in vivo implantation. As it requires significant amounts of time for vascular infiltration by the host, …
Influence Of Traumatic Impaction And Pathological Loading On Knee Menisci, Megan Leigh Killian
Influence Of Traumatic Impaction And Pathological Loading On Knee Menisci, Megan Leigh Killian
Dissertations, Master's Theses and Master's Reports - Open
Nearly half of the US population faces the risk of developing knee osteoarthritis (OA). Both in vitro and in vivo studies can aid in a better understanding of the etiology, progression, and advancement of this debilitating disorder. The knee menisci are fibrocartilagenous structures that aid in the distribution of load, attenuation of shock, alignment and lubrication of the knee. Little is known about the biochemical and morphological changes associated with knee menisci following altered loading and traumatic impaction, and investigations are needed to further elucidate how degradation of this soft tissue advances over time. The biochemical response of porcine meniscal …