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Full-Text Articles in Engineering
A Simple Apparatus For Measuring Cell Settling Velocity, Zhaowei Wang, Joanne M. Belovich
A Simple Apparatus For Measuring Cell Settling Velocity, Zhaowei Wang, Joanne M. Belovich
Chemical & Biomedical Engineering Faculty Publications
Accurate cell settling velocity determination is critical for perfusion culture using a gravity settler for cell retention. We have developed a simple apparatus (a 'settling column') for measuring settling velocity and have validated the procedure with 15-μm polystyrene particles with known physical properties. The measured settling velocity of the polystyrene particles is within 4% of the value obtained using the traditional Stokes' law approach. The settling velocities of three hybridoma cell lines were measured, resulting in up to twofold variation among cell lines, and the values decreased as the cell culture aged. The settling velocities of the nonviable cells were …
Two Approaches For Cell Retention In Perfusion Culture Systems, Zhaowei Wang
Two Approaches For Cell Retention In Perfusion Culture Systems, Zhaowei Wang
ETD Archive
A lack of efficient, economical, and reliable cell retention devices has limited the application of perfusion culture systems in the biopharmaceutical industry. Two types of cell retention devices were developed in this work for long-term perfusion culture systems: a modification of an inclined gravity settler and a variation of an ultrasonic filter. Both bench-top and large-scale tests showed that the gravity settler can effectively retain viable cells and preferentially remove nonviable cells in perfusion culture systems. The viable cell retention rate can be maintained well above 90 during long-term perfusion culture period while the nonviable cell retention rate is 20-30 …