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Biomedical Engineering and Bioengineering

California Polytechnic State University, San Luis Obispo

Master's Theses

Theses/Dissertations

2015

Cellulose

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Characterization Of Electrophorectic Separations On A Cellulose Paper-Microfluidic Chip, Kyle Robert Fast Sep 2015

Characterization Of Electrophorectic Separations On A Cellulose Paper-Microfluidic Chip, Kyle Robert Fast

Master's Theses

The purpose of this thesis project is to demonstrate the ability to utilize electrophoresis in a cellulose paper microfluidic chip to manipulate charged particles. Materials were selected and a manufacturing protocol was created to successfully apply the electric field onto the paper chip. Experiments were performed to characterize the separation rates for charged, colorimetric dye, Orange G in the membrane as a function of an applied electric field, dye concentration, and distance traveled. The experiments confirmed that the electric field can be applied to the chip and particle separation rates were characterized. Next, the determined rates results were used to …