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Adsorption Of Bovine Serum Albumin Onto Octacalcium Phosphate And Its Hydrolyzates, O. Suzuki, H. Yagishita, M. Yamazaki, T. Aoba
Adsorption Of Bovine Serum Albumin Onto Octacalcium Phosphate And Its Hydrolyzates, O. Suzuki, H. Yagishita, M. Yamazaki, T. Aoba
Cells and Materials
Octacalcium phosphate (OCP) has been advocated to be a precursor of biomineral s. Tn this report , we aimed at investigating OCP hydrolysis and the interaction of OCP and its hydrolyzates (apatitic products) with bovine serum albumin (BSA). A batch of synthetic OCP was allowed to hydrolyze either (a) in deionized water at 70°C or (b) in Tris buffer (pH 7.4) containing 2 ppm fluoride at 37°C. OCP hydrolysis was completed within reasonable experimental periods: after 48 hours at 70°C and after 10 days at 37°C in the presence of fluoride. The adsorption isotherms of BSA onto the original OCP …
Ultrastructural, Cytochemical, And Immunocytochemical Studies On Bone And Its Interfaces, M. D. Mckee, A. Nanci
Ultrastructural, Cytochemical, And Immunocytochemical Studies On Bone And Its Interfaces, M. D. Mckee, A. Nanci
Cells and Materials
Bone cells possess the ability to synthesize, secrete and direct the assembly and maintenance of extracellular matrix to form a functionally rigid and/or weight-bearing mineralized tissue complex, the skeleton. The skeleton not only supports and protects the organs and tissues of the body, but also acts as a mineral ion reservoir for maintaining systemic calcium balance (calcium homeostasis). The remarkable biological precision necessary for the formation, turnover and constant adaptation of bone to external mechanical forces is a dynamic process requiring a coordinated cellular effort relying on a variety of cell-cell and cell-matrix/mineral interactions. Indeed, cell-matrix interfaces found in a …
A Scanning Electron Microscope Study Of The Ultrastructural Organization Of Bone Mineral, F. B. Bagambisa, U. Joos, W. Schilli
A Scanning Electron Microscope Study Of The Ultrastructural Organization Of Bone Mineral, F. B. Bagambisa, U. Joos, W. Schilli
Cells and Materials
Scanning electron microscopy (SEM) was used to study samples of lamellar bone at magnifications typical for the published transmission electron micrographs, to gain more insight into the three-dimensional ultrastructure of bone mineral. Untreated (whole bone) samples allowed an assessment of the degree of mineralization. Deproteinized samples revealed the ultrastructural form and organization of bone apatite to be a function of the extent to which collagen fibers were imbibed with mineral. Numerous parallel formations reminiscent of troughs, gutters, or furrows, pierced and traversed the mineralization front. These troughs showed varying diameters identifiable with collagen fibers, and were separated from one another …