Engineering Commons^™

Development And Characterization Of Shear Thinning Cnf-Tempo Hydrogels For Use In Aquaculture Vaccines, Jacob A. Holbrook

Electronic Theses and Dissertations

Aquaculture has shown significant growth within the past few decades and is expected to continue expanding rapidly as the global demand for fish consumption continues to increase. A significant loss within the aquaculture industry is due to pathogen transmission within the farm systems. These pathogens are mitigated through various methods that are not cost effective or have limited protection. The most common method of pathogen mitigation is through oil-based injection vaccines as they provide even protection across vaccinated fish and provides the longest protection compared to other vaccination methods. These oil-based injection vaccines are not without their flaws as they …

Recent Advances In Antimicrobial Peptide Hydrogels, Aryanna Copling, Maxwell Akantibila, Raaha Kumaresan, Gilbert Fleischer, Dennise Cortes, Rahul S Tripathi, Valerie J. Carabetta, Sebastian Vega

Cooper Medical School of Rowan University Faculty Scholarship

Advances in the number and type of available biomaterials have improved medical devices such as catheters, stents, pacemakers, prosthetic joints, and orthopedic devices. The introduction of a foreign material into the body comes with a risk of microbial colonization and subsequent infection. Infections of surgically implanted devices often lead to device failure, which leads to increased patient morbidity and mortality. The overuse and improper use of antimicrobials has led to an alarming rise and spread of drug-resistant infections. To overcome the problem of drug-resistant infections, novel antimicrobial biomaterials are increasingly being researched and developed. Hydrogels are a class of 3D …

Development Of Fibroblast/Endothelial Cell-Seeded Collagen Scaffolds For In Vitro Prevascularization, Daniela S. Masson-Meyers, Fahimeh Tabatabaei, Lane Steinhaus, Jeffrey M. Toth, Lobat Tayebi

Biomedical Engineering Faculty Research and Publications

The development of vascularized scaffolds remains one of the major challenges in tissue engineering, and co-culturing with endothelial cells is known as one of the possible approaches for this purpose. In this approach, optimization of cell culture conditions, scaffolds, and fabrication techniques is needed to develop tissue equivalents that will enable in vitro formation of a capillary network. Prevascularized equivalents will be more physiologically comparable to the native tissues and potentially prevent insufficient vascularization after implantation. This study aimed to culture human umbilical vein endothelial cells (HUVECs), alone or in co-culture with fibroblasts, on collagen scaffolds prepared by simple fabrication …

Development Of A Cell-Based Regenerative Strategy To Modulate Angiogenesis And Inflammation In Ischemic Muscle, Fiona E. Serack

Electronic Thesis and Dissertation Repository

The delivery of human adipose-derived stromal cells (hASCs) to ischemic tissues represents a promising strategy to promote vascular regeneration for patients with critical limb ischemia (CLI). This thesis focused on the evaluation of hydrogels to enhance the retention and pro-angiogenic capacity of hASCs following delivery in vivo. Additionally, priming strategies to augment the paracrine function of hASCs were developed and assessed.

Recognizing the importance of endogenous macrophages in the pro-regenerative function of hASCs, delivery using a previously-developed hydrogel system, composed of peptide-functionalized methacrylated glycol chitosan (MGC-RGD) and a copolymer of poly(ethylene glycol) and poly(trimethylene carbonate) (PEG(PTMC-A)₂), was …