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Full-Text Articles in Engineering
Scanning Electron Microscopy Of The Osteoclast-Bone Interface In Vivo, H. Zhou, R. Chernecky, J. E. Davies
Scanning Electron Microscopy Of The Osteoclast-Bone Interface In Vivo, H. Zhou, R. Chernecky, J. E. Davies
Cells and Materials
Rat femoral bones were studied by scanning electron microscopy to demonstrate the morphology of osteoclast- bone matrix interfacial relationships. Two general morphological types of actively resorbing osteoclasts were observed . One cell type was approximately ovoid with highly fimbriated borders, fully attached and closely adapted to the resorption surface. The ruffled border of such cells was composed of a number of individual filopodia which were of uniform , regular shape and approximately 2-3 μm in length and 150 nm in diameter. They were found to penetrate the bone matrix to a depth of 1 μm and interdigitated with the surrounding …
The Effect Of Substratum Roughness On Osteoclast-Like Cells In Vitro, K. Gomi, J. D. De Bruijn, M. Ogura, J. E. Davies
The Effect Of Substratum Roughness On Osteoclast-Like Cells In Vitro, K. Gomi, J. D. De Bruijn, M. Ogura, J. E. Davies
Cells and Materials
Calcium phosphate powders were used to produce three groups of experimental substrata for the culture of primary rat bone marrow cells in conditions which permitted the survival and function of osteoclasts. Each of the three experimental groups were subdivided by differences in substratum surface roughness and following a culture period of 7 to 11 days, the culture units were stained for tartrate-resistant acid phosphatase activity. In all samples both small, sometimes mononuclear, and large multinucleate cells stained positive for tartrate-resistant acid phosphatase activity and the numbers and types of cells were quantified and statistically analyzed. Following histochemical staining the samples …
Osteoclastic Resorption Of Calcium Phosphate Ceramic Thin Films, J. E. Davies, G. Shapiro, B. F. Lowenberg
Osteoclastic Resorption Of Calcium Phosphate Ceramic Thin Films, J. E. Davies, G. Shapiro, B. F. Lowenberg
Cells and Materials
Sub-micron calcium phosphate ceramic thin films were formed by vertically dipping transparent quartz plates in a particulate sol-gel suspension. Primary adult rat bone marrow cell populations were cultured on the ceramic thin films in conditions known to allow the differentiation of cells of the osteoclast lineage. Monitoring the cultures for periods of 11 to 28 days revealed the creation of resorption lacunae in the thin films by multinucleate cells. Some cultures were heated at 42 °C overnight to remove adherent cells; using bright field light microscopy (LM), after staining with silver nitrate, the degree of resorption could be easily assessed. …